Circulating anti-filamin C autoantibody as a potential serum biomarker for low-grade gliomas.
Circulating anti-filamin C autoantibody as a potential serum biomarker for low-grade gliomas.
复制标题
循环抗纤维素C自身抗体作为低级神经胶质瘤的潜在血清生物标志物。
DOI：
10.1186/1471-2407-14-452
复制
发表时间：
2014-06-18
期刊：
影响因子：
3.8
通讯作者：
Iwadate Y
中科院分区：
文献类型：
--
作者：
Adachi-Hayama M;Adachi A;Shinozaki N;Matsutani T;Hiwasa T;Takiguchi M;Saeki N;Iwadate Y
脑胶质瘤是成人最常见的原发性中枢神经系统恶性肿瘤，由于其侵袭性，通常无法治愈。建立脑胶质瘤的血清学标志物有助于脑胶质瘤的早期诊断和有效治疗。细丝蛋白是一种肌动蛋白交联剂，细丝蛋白C（FLNC）通常仅限于肌肉组织，为许多信号分子提供了一个重要的通信领域。最近，film已被认为是重要的肿瘤发生的癌症。我们利用重组cDNA表达克隆（SEREX）通过抗原的血清学鉴定寻找新的胶质瘤相关抗原，并发现FLNC作为候选蛋白。采用免疫组化和定量RT-PCR方法检测FLNC在正常组织和肿瘤组织中的表达。用ELISA法检测正常人和不同级别胶质瘤患者血清中抗FLNC自身抗体水平。FLNC在胶质瘤组织中有表达，且随肿瘤分级的增高表达水平增高。抗FLNC自身抗体在胶质瘤患者血清中也有表达，但其水平与组织表达呈负相关。低级别胶质瘤患者的血清抗FLNC自身抗体水平显著高于高级别胶质瘤患者或正常志愿者，这在独立验证的患者血清中得到证实。脑膜瘤和脑梗死患者的自身抗体水平与正常人相当，但明显低于低级别胶质瘤患者。总IgG和抗谷胱甘肽S-转移酶（GST）抗体水平在患者组中没有改变，这表明自身抗体反应对FLNC具有特异性。提示血清抗FLNC自身抗体可作为低级别胶质瘤早期诊断的潜在血清学标志物，但尚需大规模的临床研究。
Glioma is the most common primary malignant central nervous system tumor in adult, and is usually not curable due to its invasive nature. Establishment of serum biomarkers for glioma would be beneficial both for early diagnosis and adequate therapeutic intervention. Filamins are an actin cross-linker and filamin C (FLNC), normally restricted in muscle tissues, offers many signaling molecules an essential communication fields. Recently, filamins have been considered important for tumorigenesis in cancers. We searched for novel glioma-associated antigens by serological identification of antigens utilizing recombinant cDNA expression cloning (SEREX), and found FLNC as a candidate protein. Tissue expressions of FLNC (both in normal and tumor tissues) were examined by immunohistochemistry and quantitative RT-PCR analyses. Serum anti-FLNC autoantibody level was measured by ELISA in normal volunteers and in the patients with various grade gliomas. FLNC was expressed in glioma tissues and its level got higher as tumor grade advanced. Anti-FLNC autoantibody was also detected in the serum of glioma patients, but its levels were inversely correlated with the tissue expression. Serum anti-FLNC autoantibody level was significantly higher in low-grade glioma patients than in high-grade glioma patients or in normal volunteers, which was confirmed in an independent validation set of patients’ sera. The autoantibody levels in the patients with meningioma or cerebral infarction were at the same level of normal volunteers, and they were significantly lower than that of low-grade gliomas. Total IgG and anti-glutatione S-transferase (GST) antibody level were not altered among the patient groups, which suggest that the autoantibody response was specific for FLNC. The present results suggest that serum anti-FLNC autoantibody can be a potential serum biomarker for early diagnosis of low-grade gliomas while it needs a large-scale clinical study.
登录
查看更多内容
DOI：
10.1186/1756-9966-31-85
发表时间：
2012-10-11
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Matsutani T;Hiwasa T;Takiguchi M;Oide T;Kunimatsu M;Saeki N;Iwadate Y
通讯作者：
Iwadate Y
DOI：
10.1158/1078-0432.ccr-08-1402
发表时间：
2009-02-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Bedolla RG;Wang Y;Asuncion A;Chamie K;Siddiqui S;Mudryj MM;Prihoda TJ;Siddiqui J;Chinnaiyan AM;Mehra R;de Vere White RW;Ghosh PM
通讯作者：
Ghosh PM
影响因子：
8
作者：
Ueda, R;Iizuka, Y;Toda, M
通讯作者：
Toda, M
影响因子：
9.2
作者：
Jiang X;Yue J;Lu H;Campbell N;Yang Q;Lan S;Haffty BG;Yuan C;Shen Z
通讯作者：
Shen Z
DOI：
10.1073/pnas.96.14.7932
发表时间：
1999-07-06
影响因子：
11.1
作者：
Schwarzman, AL;Singh, N;Goldgaber, D
通讯作者：
Goldgaber, D