JAGGED controls growth anisotropyand coordination between cell sizeand cell cycle during plant organogenesis.
JAGGED controls growth anisotropyand coordination between cell sizeand cell cycle during plant organogenesis.
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DOI：
10.1016/j.cub.2012.07.020
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发表时间：
2012-10-09
期刊：
影响因子：
9.2
通讯作者：
Sablowski, Robert
中科院分区：
文献类型：
--
作者：
Schiessl, Katharina;Kausika, Swathi;Southam, Paul;Bush, Max;Sablowski, Robert
在所有多细胞生物中，模式基因、细胞生长、细胞周期、细胞大小稳态和器官生长之间的联系尚不清楚，部分原因是在生长器官中对细胞行为进行动态、三维分析的困难。植物器官发生的关键步骤是从顶端分生组织中产生器官原基。在这里，我们结合细胞几何和DNA合成的定量，三维分析来研究转录因子JAGGED （JAG）的作用，它在拟南芥花的图案和原基生长之间的界面起作用。花分生组织呈各向同性生长，细胞体积与DNA合成紧密协调。萼片原基加速了细胞分裂、细胞增大、各向异性生长和DNA合成与细胞体积的解耦，同时增加了细胞大小的异质性。所有这些生长参数的变化都需要JAG，并且与原基萌发在遗传上是可分离的。分生组织中异位的JAG活性促进了以不适当的小细胞体积进入S期，这表明JAG可以覆盖分生组织中起作用的细胞大小检查点。JAG在花发育过程中直接抑制分生组织调控基因BREVIPEDICELLUS和BELL 1，这与分生组织向原基身份转变的作用一致。我们定义了从分生组织到器官身份转变的细胞基础，并确定JAG是这一转变的关键调节因子。JAG促进各向异性生长，是与器官原基加速生长和分化相关的细胞大小动态平衡变化所必需的。►JAGGED （JAG）是拟南芥初始花器官生长的必需基因►JAG在器官萌发过程中使细胞周期与细胞生长脱钩►当花器官从分生组织萌发时，JAG促进快速、各向异性生长►JAG直接抑制分生组织身份基因
In all multicellular organisms, the links between patterning genes, cell growth, cell cycle, cell size homeostasis, and organ growth are poorly understood, partly due to the difficulty of dynamic, 3D analysis of cell behavior in growing organs. A crucial step in plant organogenesis is the emergence of organ primordia from the apical meristems. Here, we combined quantitative, 3D analysis of cell geometry and DNA synthesis to study the role of the transcription factor JAGGED (JAG), which functions at the interface between patterning and primordium growth in Arabidopsis flowers. The floral meristem showed isotropic growth and tight coordination between cell volume and DNA synthesis. Sepal primordia had accelerated cell division, cell enlargement, anisotropic growth, and decoupling of DNA synthesis from cell volume, with a concomitant increase in cell size heterogeneity. All these changes in growth parameters required JAG and were genetically separable from primordium emergence. Ectopic JAG activity in the meristem promoted entry into S phase at inappropriately small cell volumes, suggesting that JAG can override a cell size checkpoint that operates in the meristem. Consistent with a role in the transition from meristem to primordium identity, JAG directly repressed the meristem regulatory genes BREVIPEDICELLUS and BELL 1 in developing flowers. We define the cellular basis for the transition from meristem to organ identity and identify JAG as a key regulator of this transition. JAG promotes anisotropic growth and is required for changes in cell size homeostasis associated with accelerated growth and the onset of differentiation in organ primordia. ► JAGGED (JAG) is required for growth of initiating floral organs in Arabidopsis ► JAG decouples cell cycle from cell growth during organ emergence ► JAG promotes fast, anisotropic growth when floral organs emerge from the meristem ► JAG directly represses meristem identity genes
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影响因子：
4.6
作者：
Dinneny, JR;Yadegari, R;Weigel, D
通讯作者：
Weigel, D
影响因子：
9.8
作者：
Laskowski M;Grieneisen VA;Hofhuis H;Hove CA;Hogeweg P;Marée AF;Scheres B
通讯作者：
Scheres B
影响因子：
9.8
作者：
Kaufmann K;Muiño JM;Jauregui R;Airoldi CA;Smaczniak C;Krajewski P;Angenent GC
通讯作者：
Angenent GC
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
6.9
作者：
Kwiatkowska, D;Dumais, J
通讯作者：
Dumais, J