Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy.
Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy.
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DOI：
10.1002/jemt.20757
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发表时间：
2010-01
影响因子：
2.5
通讯作者：
Lawson, Jeffrey H.
中科院分区：
文献类型：
--
作者：
Achneck, Hardean E.;Serpe, Michael J.;Jamiolkowski, Ryan M.;Eibest, Leslie M.;Craig, Stephen L.;Lawson, Jeffrey H.
钛是人体植入器械最常用的材料之一。扫描电子显微镜（SEM）是对钛表面进行成像并分析粘附在钛植入物上的细胞和其他有机物的重要工具。然而，非导电样品的高真空SEM成像需要表面上的导电涂层。通常使用金/钯涂层，并且迄今为止还没有描述在不蚀刻钛本身的情况下“清洁”这种金/钯覆盖的表面以用于重复实验的方法。这构成了钛基可植入装置的主要问题，钛基可植入装置非常昂贵，因此供应短缺。我们的目标是设计一个协议，再生钛表面后，SEM分析。在一系列实验中，将来自可植入心脏辅助装置的钛样品用纤连蛋白涂覆，用细胞接种，然后用金/钯涂覆用于SEM分析。在五种不同的清洁方案之前和之后获得X射线光电子能谱。用王水（浓硝酸和盐酸的1：3溶液）处理，有或没有臭氧分解，然后在肥皂溶液中超声处理和在去离子水中超声处理，使钛表面再生到其原始状态。原子力显微镜证实，建立的协议没有改变钛的微观结构。本文所述的方案适用于生物医学科学中使用的几乎所有钛表面，并且由于其暴露于王水的时间短，可能也适用于许多钛合金。
Titanium is one of the most commonly used materials for implantable devices in human s. Scanning electron microscopy (SEM) serves as an important tool for imaging titanium surfaces and analyzing cells and other organic matter adhering to titanium implants. However, high-vacuum SEM imaging of a non-conductive sample requires a conductive coating on the surface. A gold/ palladium coating is commonly used and to date no method has been described to ‘clean’ such gold/ palladium covered surfaces for repeated experiments without etching the titanium itself. This constitutes a major problem with titanium based implantable devices which are very expensive and thus in short supply. Our objective was to devise a protocol to regenerate titanium surfaces after SEM analysis. In a series of experiments, titanium samples from implantable cardiac assist devices were coated with fibronectin, seeded with cells and then coated with gold/palladium for SEM analysis. X-ray photoelectron spectroscopy spectra were obtained before and after five different cleaning protocols. Treatment with aqua regia (a 1:3 solution of concentrated nitric and hydrochloric acid), with or without ozonolysis, followed by sonication in soap solution and sonication in deionized water, allowed regenerating titanium surfaces to their original state. Atomic force microscopy confirmed that the established protocol did not alter the titanium microstructure. The protocol described herein is applicable to almost all titanium surfaces used in biomedical sciences and because of its short exposure time to aqua regia, will likely work for many titanium alloys as well.
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影响因子：
2.1
作者：
Parashos, P;Linsuwanont, P;Messer, HH
通讯作者：
Messer, HH
影响因子：
1.2
作者：
Takahashi, Kazuhiro;Fukuzaki, Satoshi
通讯作者：
Fukuzaki, Satoshi
影响因子：
1.9
作者：
BETZ, G
通讯作者：
BETZ, G
影响因子：
3
作者：
Breithaupt-Faloppa AC;de Lima WT;Oliveira-Filho RM;Kleinheinz J
通讯作者：
Kleinheinz J
影响因子：
20.1
作者：
Werner, N;Junk, S;Nickenig, G
通讯作者：
Nickenig, G