Comparative Transcriptome Analysis Revealing the Different Germination Process in Aryloxyphenoxypropionate-Resistant and APP-Susceptible Asia Minor Bluegrass (Polypogon fugax).
Comparative Transcriptome Analysis Revealing the Different Germination Process in Aryloxyphenoxypropionate-Resistant and APP-Susceptible Asia Minor Bluegrass (Polypogon fugax).
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比较转录组分析揭示了抗芳氧基苯氧基丙酸酯和 APP 敏感的小亚细亚早熟禾 (Polypogon fugax) 的不同发芽过程
DOI：
10.3390/plants9091191
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发表时间：
2020-09-12
期刊：
影响因子：
--
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Yu X;Tang W;Yang Y;Zhang J;Lu Y
预测除草剂抗性突变在无除草剂条件下表现出适应度成本。小亚细亚早熟禾（Polypogon fugax）是冬种作物上常见的杂草。我们之前的研究建立了一个对芳氧基苯氧基丙酸酯（APP）除草剂具有抗性的黑腐病菌种属（LR），其相对于敏感种属（LS）也表现出萌发延迟。进行比较转录组以分析在两个萌发时间点的LS和LR的基因表达谱。在LS和LR中分别鉴定出11，856和23，123个差异表达基因（DEG）。大部分DEG参与脂质代谢、碳水化合物代谢、氨基酸代谢和次生代谢物的生物合成。24个参与碳水化合物和脂肪酸代谢的基因在萌发过程中LS的相对表达水平高于LR。9个参与赤霉素（GA）和脱落酸（阿坝）信号转导的基因在LS和LR中表现出不同的表达模式，这与它们对外源激素处理的不同敏感性相一致。本研究首次揭示了白松种子萌发过程中转录水平的变化及其相互作用。比较了APP除草剂抗性和敏感材料在萌发过程中的差异表达谱，有助于理解除草剂抗性与适合度代价之间的关系。
Herbicide-resistant mutations are predicted to exhibit fitness cost under herbicide-free conditions. Asia minor bluegrass (Polypogon fugax) is a common weed species in the winter crops. Our previous study established a P. fugax accession (LR) resistant to aryloxyphenoxypropionate (APP) herbicides, which also exhibited germination delay relative to the susceptible accession (LS). A comparative transcriptome was conducted to analyze the gene expression profile of LS and LR at two germination time points. A total of 11,856 and 23,123 differentially expressed genes (DEGs) were respectively identified in LS and LR. Most DEGs were involved in lipid metabolism, carbohydrate metabolism, amino acid metabolism, and secondary metabolites biosynthesis. Twenty-four genes involved in carbohydrate and fatty acid metabolism had higher relative expression levels in LS than LR during germination. Nine genes involved in gibberellin (GA) and abscisic acid (ABA) signal transduction showed different expression patterns in LS and LR, consistent with their different sensitivity to exogenous hormones treatments. This study first provided insight into transcriptional changes and interaction in the seed germination process of P. fugax. It compared the differential expression profile between APP herbicides resistance and susceptible accessions during germination, which contributed to understanding the association between herbicide resistance and fitness cost.
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影响因子：
10.5
作者：
Lee, SC;Cheng, H;Peng, JR
通讯作者：
Peng, JR
影响因子：
7.4
作者：
Lindgren, LO;Stålberg, KG;Höglund, AS
通讯作者：
Höglund, AS
影响因子：
5.6
作者：
Frenkel E;Matzrafi M;Rubin B;Peleg Z
通讯作者：
Peleg Z
DOI：
10.1073/pnas.91.17.8209
发表时间：
1994-08-16
影响因子：
11.1
作者：
DALDEGAN, F;ROCHER, A;VON WETTSTEIN, D
通讯作者：
VON WETTSTEIN, D
影响因子：
4.2
作者：
Delye, Christophe;Menchari, Yosra;Le Corre, Valerie
通讯作者：
Le Corre, Valerie