Lyophilized plasma attenuates vascular permeability, inflammation and lung injury in hemorrhagic shock.
Lyophilized plasma attenuates vascular permeability, inflammation and lung injury in hemorrhagic shock.
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DOI：
10.1371/journal.pone.0192363
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Kozar RA
中科院分区：
文献类型：
--
作者：
Pati S;Peng Z;Wataha K;Miyazawa B;Potter DR;Kozar RA
在严重创伤和出血时，早期和经验性使用新鲜冷冻血浆（FFP）可降低发病率和死亡率。然而，FFP的使用伴随着冷冻血液制品的运输和储存的重大负担。干燥或冻干血浆（LP）可在室温下储存，易于运输，快速复溶，在偏远和严峻的环境中随时可用。我们以前已经证明，FFP减轻了出血性休克（HS）后的内皮损伤。在目前的研究中，我们试图确定LP是否具有与FFP相似的特性，在体外和体内调节内皮功能障碍的能力。使用以下体外内皮细胞（EC）功能指标比较单供体LP和单供体FFP：渗透性和跨内皮单层阻力;粘附连接保存;和白细胞-EC粘附。在体内，使用鼠HS模型，比较LP和FFP在HS诱导的肺血管炎症和水肿的测量中的作用。无论是在体外和体内的EC功能的所有措施，LP表现出类似的效果FFP。FFP和LP同样降低EC渗透性，增加跨内皮阻力，降低白细胞-EC结合和持久的粘附连接。在体内，LP和FFP均能显著减轻肺损伤、炎症和血管渗漏。FFP和LP在体外对血管内皮和在体对失血性休克后肺功能具有相似的有效保护作用。这些数据支持进一步开发LP作为创伤和失血性休克后人体使用的有效血浆产品。
In severe trauma and hemorrhage the early and empiric use of fresh frozen plasma (FFP) is associated with decreased morbidity and mortality. However, utilization of FFP comes with the significant burden of shipping and storage of frozen blood products. Dried or lyophilized plasma (LP) can be stored at room temperature, transported easily, reconstituted rapidly with ready availability in remote and austere environments. We have previously demonstrated that FFP mitigates the endothelial injury that ensues after hemorrhagic shock (HS). In the current study, we sought to determine whether LP has similar properties to FFP in its ability to modulate endothelial dysfunction in vitro and in vivo. Single donor LP was compared to single donor FFP using the following measures of endothelial cell (EC) function in vitro: permeability and transendothelial monolayer resistance; adherens junction preservation; and leukocyte-EC adhesion. In vivo, using a model of murine HS, LP and FFP were compared in measures of HS- induced pulmonary vascular inflammation and edema. Both in vitro and in vivo in all measures of EC function, LP demonstrated similar effects to FFP. Both FFP and LP similarly reduced EC permeability, increased transendothelial resistance, decreased leukocyte-EC binding and persevered adherens junctions. In vivo, LP and FFP both comparably reduced pulmonary injury, inflammation and vascular leak. Both FFP and LP have similar potent protective effects on the vascular endothelium in vitro and in lung function in vivo following hemorrhagic shock. These data support the further development of LP as an effective plasma product for human use after trauma and hemorrhagic shock.
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DOI：
10.1097/ta.0b013e318227edbb
发表时间：
2011-08-01
影响因子：
--
作者：
Holcomb, John B.;Zarzabal, Lee A.;Wade, Charles E.
通讯作者：
Wade, Charles E.
影响因子：
3.4
作者：
Peng, Zhanglong;Pati, Shibani;Kozar, Rosemary A.
通讯作者：
Kozar, Rosemary A.
影响因子：
2.9
作者：
Lee, Tim H.;Van, Philbert Y.;Schreiber, Martin A.
通讯作者：
Schreiber, Martin A.
DOI：
10.1097/shk.0b013e31829f91fc
发表时间：
2013-09
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Peng Z;Pati S;Potter D;Brown R;Holcomb JB;Grill R;Wataha K;Park PW;Xue H;Kozar RA
通讯作者：
Kozar RA
影响因子：
9
作者：
Cotton BA;Reddy N;Hatch QM;LeFebvre E;Wade CE;Kozar RA;Gill BS;Albarado R;McNutt MK;Holcomb JB
通讯作者：
Holcomb JB