Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.
Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.
复制标题
通过逆转刺激极性来取消细胞对纳米电的反应。
DOI：
10.1007/s00018-014-1626-z
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发表时间：
2014-11
影响因子：
8
通讯作者：
Ibey, Bennett L.
中科院分区：
文献类型：
--
作者：
Pakhomov, Andrei G.;Semenov, Iurii;Xiao, Shu;Pakhomova, Olga N.;Gregory, Betsy;Schoenbach, Karl H.;Ullery, Jody C.;Beier, Hope T.;Rajulapati, Sambasiva R.;Ibey, Bennett L.
关键词：
生物膜的纳米电穿孔是高电压、纳秒持续时间的电脉冲（nsEP）的效应。它发生在质膜和细胞内，纳米孔膜的特点是离子选择性和电位敏感的渗透性。在这里，我们报告了一种新的现象，生物效应取消，把nsEP基本上除了传统的电穿孔和电刺激的毫秒和微秒脉冲。我们比较了60-和300-ns的单极，近矩形nsEP对细胞内Ca 2+动员和细胞存活与双极60 + 60和300 + 300 ns脉冲的影响。对于不同的终点、暴露条件、脉冲数（1-60）和振幅（15-60 kV/cm），添加第二阶段抵消了第一阶段的影响。双极脉冲的整体效果大大降低，尽管提供了两倍的能量。当两相分离成两个独立的相反极性的nsEP时，也会发生抵消;随着两个nsEP之间的间隔增加，抵消逐渐减弱，但即使在10 μs的间隔下仍然存在。这种抵消现象是nsEP所特有的，并且还没有被电穿孔的等效电路、输运晶格和分子动力学模型所预测。需要修改现有的nsEP膜透化范例。在这里，我们讨论了可能参与的辅助膜放电，膜磷脂的两步氧化，和反向跨膜离子转运机制。取消影响癌症治疗，电刺激和生物技术中的nsEP应用，并提供了更复杂的波形，包括脉冲电磁发射的影响的新见解。
Nanoelectroporation of biomembranes is an effect of high-voltage, nanosecond-duration electric pulses (nsEP). It occurs both in the plasma membrane and inside the cell, and nanoporated membranes are distinguished by ion-selective and potential-sensitive permeability. Here we report a novel phenomenon of bioeffects cancellation that puts nsEP cardinally apart from the conventional electroporation and electrostimulation by milli- and microsecond pulses. We compared the effects of 60- and 300-ns monopolar, nearly rectangular nsEP on intracellular Ca2+ mobilization and cell survival with those of bipolar 60 + 60 and 300 + 300 ns pulses. For diverse endpoints, exposure conditions, pulse numbers (1–60), and amplitudes (15–60 kV/cm), the addition of the second phase cancelled the effects of the first phase. The overall effect of bipolar pulses was profoundly reduced, despite delivering twofold more energy. Cancellation also took place when two phases were separated into two independent nsEP of opposite polarities; it gradually tapered out as the interval between two nsEP increased, but was still present even at a 10-μs interval. The phenomenon of cancellation is unique for nsEP and has not been predicted by the equivalent circuit, transport lattice, and molecular dynamics models of electroporation. The existing paradigms of membrane permeabilization by nsEP will need to be modified. Here we discuss the possible involvement of the assisted membrane discharge, two-step oxidation of membrane phospholipids, and reverse transmembrane ion transport mechanisms. Cancellation impacts nsEP applications in cancer therapy, electrostimulation, and biotechnology, and provides new insights into effects of more complex waveforms, including pulsed electromagnetic emissions.
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DOI：
10.1016/j.bbrc.2013.12.004
发表时间：
2014-01-10
影响因子：
3.1
作者：
Ibey, Bennett L.;Ullery, Jody C.;Pakhomova, Olga N.;Roth, Caleb C.;Semenov, Iurii;Beier, Hope T.;Tarango, Melissa;Xiao, Shu;Schoenbach, Karl H.;Pakhomov, Andrei G.
通讯作者：
Pakhomov, Andrei G.
影响因子：
3
作者：
Ibey, Bennett L.;Pakhomov, Andrei G.;Gregory, Betsy W.;Khorokhorina, Vera A.;Roth, Caleb C.;Rassokhin, Mikhail A.;Bernhard, Joshua A.;Wilmink, Gerald J.;Pakhomova, Olga N.
通讯作者：
Pakhomova, Olga N.
影响因子：
3.9
作者：
Arena CB;Sano MB;Rossmeisl JH Jr;Caldwell JL;Garcia PA;Rylander MN;Davalos RV
通讯作者：
Davalos RV
影响因子：
4.6
作者：
Arena, Christopher B.;Sano, Michael B.;Davalos, Rafael V.
通讯作者：
Davalos, Rafael V.
影响因子：
2.4
作者：
Joshi, RP;Schoenbach, KH
通讯作者：
Schoenbach, KH