MicroRNA-mediated mRNA translation activation in quiescent cells and oocytes involves recruitment of a nuclear microRNP.
MicroRNA-mediated mRNA translation activation in quiescent cells and oocytes involves recruitment of a nuclear microRNP.
复制标题
DOI：
10.1038/srep00842
复制
发表时间：
2012
影响因子：
4.6
通讯作者：
Vasudevan, S.
中科院分区：
文献类型：
--
作者：
Truesdell, S. S.;Mortensen, R. D.;Seo, M.;Schroeder, J. C.;Lee, J. H.;LeTonqueze, O.;Vasudevan, S.
MicroRNA可以促进静止期（G 0）哺乳动物细胞和未成熟非洲爪蟾卵母细胞中特定mRNA的翻译。我们报道了microRNA介导的卵母细胞中靶mRNA的上调依赖于microRNA的核进入;细胞质注射的microRNA抑制靶mRNA。激活microRNP的组分AGO、FXR 1（FXR 1-iso-a）和miR 16存在于细胞核和细胞质中。重要的是，用于上调的microRNA靶mRNA、Myt 1、TNFα和携带富含TNFα AU的microRNA靶序列的报告基因分别与未成熟卵母细胞核中的AGO和G 0人类核中的AGO 2相关。被抑制或缺乏靶位点的mRNA与核AGO无关。交联偶联免疫纯化显示AGO 2与FXR 1在细胞核中的关联性大于细胞质。一致地，FXR 1-iso-a的过表达挽救了细胞质注射的RNA的激活和低密度的增殖细胞。这些数据表明了区室化AGO 2-FXR 1-iso-a复合物对于选择性募集microRNA介导的上调的重要性。
MicroRNAs can promote translation of specific mRNAs in quiescent (G0) mammalian cells and immature Xenopus laevis oocytes. We report that microRNA-mediated upregulation of target mRNAs in oocytes is dependent on nuclear entry of the microRNA; cytoplasmically-injected microRNA repress target mRNAs. Components of the activation microRNP, AGO, FXR1 (FXR1-iso-a) and miR16 are present in the nucleus and cytoplasm. Importantly, microRNA target mRNAs for upregulation, Myt1, TNFα and a reporter bearing the TNFα AU-rich, microRNA target sequence, are associated with AGO in immature oocyte nuclei and AGO2 in G0 human nuclei, respectively. mRNAs that are repressed or lack target sites are not associated with nuclear AGO. Crosslinking-coupled immunopurification revealed greater association of AGO2 with FXR1 in the nucleus compared to cytoplasm. Consistently, overexpression of FXR1-iso-a rescues activation of cytoplasmically-injected RNAs and in low density, proliferating cells. These data indicate the importance of a compartmentalized AGO2-FXR1-iso-a complex for selective recruitment for microRNA-mediated upregulation.
登录
查看更多内容
影响因子：
2.9
作者：
Dubé M;Huot ME;Khandjian EW
通讯作者：
Khandjian EW
影响因子：
9.8
作者：
Coller HA;Sang L;Roberts JM
通讯作者：
Roberts JM
影响因子：
11.4
作者：
Ding, Xavier C.;Grosshans, Helge
通讯作者：
Grosshans, Helge
DOI：
10.1073/pnas.0811523106
发表时间：
2009-04-28
影响因子：
11.1
作者：
Hwang, Hun-Way;Wentzel, Erik A.;Mendell, Joshua T.
通讯作者：
Mendell, Joshua T.
影响因子：
10.5
作者：
Caudy, AA;Myers, M;Hammond, SM
通讯作者：
Hammond, SM