Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.
Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.
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亚细胞靶向光遗传学cAMP依赖性蛋白激酶的设计和分析。
DOI：
10.1016/j.chembiol.2017.09.011
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发表时间：
2018-01-18
影响因子：
8.6
通讯作者：
Lawrence DS
中科院分区：
文献类型：
--
作者：
O'Banion CP;Priestman MA;Hughes RM;Herring LE;Capuzzi SJ;Lawrence DS
Although the cAMP-dependent protein kinase (PKA) is ubiquitously expressed, it is sequestered at specific subcellular locations throughout the cell, thereby resulting in compartmentalized cellular signaling that triggers site-specific behavioral phenotypes. We developed a three step engineering strategy to construct an optogenetic PKA (optoPKA) and demonstrated that, upon illumination, optoPKA migrates to specified intracellular sites. Furthermore, we designed intracellular spatially segregated reporters of PKA activity and confirmed that optoPKA phosphorylates these reporters in a light-dependent fashion. Finally, proteomics experiments reveal that light activation of optoPKA results phosphorylation of known endogenous PKA substrates as well as potential novel substrates. O’Banion et al. describe the engineering, validation, and phosphoproteomics assessment of light-activated, organelle-directed, cAMP-dependent protein kinase
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光遗传凋亡：光触发的细胞死亡。
DOI：
10.1002/anie.201506346
发表时间：
2015-10-05
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Hughes RM;Freeman DJ;Lamb KN;Pollet RM;Smith WJ;Lawrence DS
通讯作者：
Lawrence DS
影响因子：
48
作者：
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通讯作者：
Tucker, Chandra L.
DOI：
10.1073/pnas.220413597
发表时间：
2000-10-24
影响因子：
11.1
作者：
Fang, XJ;Yu, SX;Mills, GB
通讯作者：
Mills, GB
影响因子：
--
作者：
Depry, Charlene;Allen, Michael D.;Zhang, Jin
通讯作者：
Zhang, Jin
影响因子：
30.8
作者：
Goh, Gerald;Scholl, Ute I.;Healy, James M.;Choi, Murim;Prasad, Manju L.;Nelson-Williams, Carol;Kuntsman, John W.;Korah, Reju;Suttorp, Anna-Carinna;Dietrich, Dimo;Haase, Matthias;Willenberg, Holger S.;Stalberg, Peter;Hellman, Per;Akerstrom, Goran;Bjorklund, Peyman;Carling, Tobias;Lifton, Richard P.
通讯作者：
Lifton, Richard P.