Immunohistochemical analysis of EGF in epiphyseal growth plate from normal, hypophysectomized, and growth hormone-treated hypophysectomized rats
Immunohistochemical analysis of EGF in epiphyseal growth plate from normal, hypophysectomized, and growth hormone-treated hypophysectomized rats
复制标题
正常、垂体切除和生长激素治疗的垂体切除大鼠骨骺生长板中 EGF 的免疫组织化学分析
作者：
Y. Tajima;Kohtaro Kato;M. Kashimata;M. Hiramatsu;N. Utsumi
采用免疫组化方法检测正常大鼠、去垂体大鼠和去垂体大鼠胫骨近端骨骺生长板软骨中表皮生长因子（EGF）的含量。在正常生长板中，EGF主要分布在增殖区。垂体切除术导致软骨细胞和软骨基质明显萎缩（成熟型软骨细胞数量减少，增生与肥大软骨细胞比例降低），EGF免疫反应性显著降低。生长激素治疗逆转了垂体切除术的这些作用，导致生长板和所有软骨细胞层中egf反应位点的厚度增加。然而，最强烈的EGF免疫染色常见于扁平外观的软骨细胞核。EGF似乎可以在有丝分裂活性显著的软骨细胞中结合或合成。目前的结果，结合之前关于EGF参与软骨组织体内和体外生长的数据，强烈表明EGF免疫反应性软骨细胞在GH的特定影响下参与软骨的增殖和生长。
Epiphyseal growth plate cartilages from the proximal tibia of normal, hypophysectomized, and growth hormone (GH)-treated hypophysectomized rats were subjected to immunohistochemistry for detection of epidermal growth factor (EGF). In the normal growth plate, EGF was distributed mainly in the proliferative zone. Hypophysectomy resulted in considerable atrophy of the chondrocytes and the cartilage matrix (a decreased number of mature-type chondrocytes and a decreased ratio of proliferating to hypertrophic chondrocytes) and a significant diminution of EGF immunoreactivity. Treatment with GH reversed these effects of hypophysectomy, causing an increased thickness of the growth plate and EGF-reactive sites in all chondrocyte layers. The most intense immunostaining for EGF, however, was frequently seen in the nuclei of chondrocytes with flattened appearance. It appears that EGF could be incorporated or synthesized in chondrocytes having marked mitogenic activity. The present results, taken with previous data on EGF involvement in growth of cartilaginous tissue in vivo and in vitro, strongly suggest that EGF-immunoreactive chondrocytes are involved in cartilage proliferation and growth under the specific influence of GH.
大鼠肝脏再生过程中表皮生长因子易位至肝细胞核。
影响因子：
29.4
作者：
Raper,SE;Burwen,SJ;Barker,ME;Jones,AL
通讯作者：
Jones,AL
带有适当表面受体的细胞中表皮生长因子、神经生长因子和血小板衍生生长因子的染色质结合。
DOI：
10.1073/pnas.83.11.3728
发表时间：
1986
影响因子：
11.1
作者：
Rakowicz-Szulczynska,EM;Rodeck,U;Herlyn,M;Koprowski,H
通讯作者：
Koprowski,H
GH 刺激与 bFGF 在无血清培养基中培养的兔软骨细胞合成的 IGF1 原型。
影响因子：
3.7
作者：
Demarquay,D;Dumontier,MF;Bourguignon,J;Hintz,RL;Corvol,MT
通讯作者：
Corvol,MT