The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL).
The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL).
复制标题
番茄测序项目，这是国际茄科项目（SOL）的第一个基石。
DOI：
10.1002/cfg.468
复制
发表时间：
2005
影响因子：
--
通讯作者：
Zamir, D
中科院分区：
文献类型：
--
作者：
Mueller, LA;Tanksley, SD;Giovannoni, JJ;van Eck, J;Stack, S;Choi, D;Kim, BD;Chen, MS;Cheng, ZK;Li, CY;Ling, HQ;Xue, YB;Seymour, G;Bishop, G;Bryan, G;Sharma, R;Khurana, J;Tyagi, A;Chattopadhyay, D;Singh, NK;Stiekema, W;Lindhout, P;Jesse, T;Lankhorst, RK;Bouzayen, M;Shibata, D;Tabata, S;Granell, A;Botella, MA;Giullano, G;Frusciante, L;Causse, M;Zamir, D
由10个国家（韩国、中国、英国、印度、荷兰、法国、日本、西班牙、意大利和美国）组成的国际财团正在对番茄（Solanum lycopersicum）的基因组进行测序，这是一项名为“国际茄科基因组计划（SOL）：多样性和适应性的系统方法”的更大倡议的一部分。2003年11月发起的这一基层倡议的目标是建立一个信息、资源和科学家网络，以最终解决植物生物学和农业中两个最重要的问题：（1）一组共同的基因/蛋白质如何产生占据我们星球的各种形态和生态上不同的生物体？(2)如何才能更深入地了解植物多样性的遗传基础，以环境友好和可持续的方式更好地满足社会的需要？Solanaceae和与之密切相关的物种，如列入SOL项目范围的咖啡，非常适合解决这两个问题。SOL项目的第一步是使用有序BAC方法生成番茄常染色质部分的高质量序列，作为茄科的参考。由于茄科中高水平的宏观和微观同线性，BAC-by-BAC番茄序列将形成用于其它物种的鸟枪测序的框架。基因组测序的起点是通过过度杂交和AFLP技术锚定到遗传图谱上的BAC。overgos来自番茄高密度F2-2000遗传图谱（http：//sgn.cornell.edu/）的大约1500个标记。这些种子BAC将被用作锚点，从该锚点使用BAC末端序列数据辐射平铺路径。将根据SOL项目指南进行注释。在SOL保护伞下产生的所有信息都将在一个综合网站上提供。这些信息将与茄科的比较生物学及其他学科的最终目标相互联系，以促进系统生物学方法的发展。
The genome of tomato (Solanum lycopersicum) is being sequenced by an international consortium of 10 countries (Korea, China, the United Kingdom, India, The Netherlands, France, Japan, Spain, Italy and the United States) as part of a larger initiative called the ‘International Solanaceae Genome Project (SOL): Systems Approach to Diversity and Adaptation’. The goal of this grassroots initiative, launched in November 2003, is to establish a network of information, resources and scientists to ultimately tackle two of the most significant questions in plant biology and agriculture: (1) How can a common set of genes/proteins give rise to a wide range of morphologically and ecologically distinct organisms that occupy our planet? (2) How can a deeper understanding of the genetic basis of plant diversity be harnessed to better meet the needs of society in an environmentally friendly and sustainable manner? The Solanaceae and closely related species such as coffee, which are included in the scope of the SOL project, are ideally suited to address both of these questions. The first step of the SOL project is to use an ordered BAC approach to generate a high quality sequence for the euchromatic portions of the tomato as a reference for the Solanaceae. Due to the high level of macro and micro-synteny in the Solanaceae the BAC-by-BAC tomato sequence will form the framework for shotgun sequencing of other species. The starting point for sequencing the genome is BACs anchored to the genetic map by overgo hybridization and AFLP technology. The overgos are derived from approximately 1500 markers from the tomato high density F2-2000 genetic map (http://sgn.cornell.edu/). These seed BACs will be used as anchors from which to radiate the tiling path using BAC end sequence data. Annotation will be performed according to SOL project guidelines. All the information generated under the SOL umbrella will be made available in a comprehensive website. The information will be interlinked with the ultimate goal that the comparative biology of the Solanaceae—and beyond—achieves a context that will facilitate a systems biology approach.
登录
查看更多内容
影响因子：
7.4
作者：
Fernie, AR;Willmitzer, L
通讯作者：
Willmitzer, L
影响因子：
4.4
作者：
Cai, WW;Reneker, J;Bradley, A
通讯作者：
Bradley, A
影响因子：
11.6
作者：
Fulton, TM;Van der Hoeven, R;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
11.6
作者：
Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
7.4
作者：
Hui, DQ;Iqbal, J;Baldwin, IT
通讯作者：
Baldwin, IT