The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection.
The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection.
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DOI：
10.3390/ijms23136971
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发表时间：
2022-06-23
影响因子：
5.6
通讯作者：
Jiang, Bo
中科院分区：
文献类型：
--
作者：
Cheng, Chunzhen;Zhong, Yun;Wang, Bin;Zhang, Yongyan;Wu, Huan;Jiang, Nonghui;Wu, Bo;Lv, Yuanda;Jiang, Bo
柑桔黄龙病对柑桔产业的威胁已持续了世纪。本研究从柑橘中克隆了一个HL B诱导的拟南芥RPM 1相互作用蛋白4（RIN 4）同源基因，并对其特征和功能进行了分析，以确定其在柑橘与黄龙病菌（CLas）相互作用中的作用。实时荧光定量PCR结果表明，RIN 4在根、茎、叶和花中均有表达，其中在叶中的表达量最高。赤霉酸、吲哚乙酸、水杨酸和茉莉酸处理抑制其表达，而脱落酸和盐处理以及创伤则诱导其表达。RIN 4-GFP的瞬时表达表明RIN 4定位于细胞膜。RIN 4过表达转基因C.马克西马湾转基因植株对CLas感染的敏感性比非转基因植株高，HLB症状出现的时间也比非转基因植株早。本研究结果表明，RIN 4在HLB病害柑橘中的表达上调可能有助于CLas的感染。
The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression was suppressed by gibberellic acid, indole-3-acetic acid, salicylic acid and jasmonic acid treatments, but was induced by abscisic acid and salt treatments, as well as wounding. The transient expression of a RIN4-GFP showed that RIN4 was localized in the cell membrane. RIN4-overexpressing transgenic C. maxima cv. ‘Shatianyou’ plants were obtained, and some transgenic plants showed greater sensitivity to CLas infection and earlier HLB symptoms appearance than non-transgenic controls. Results obtained in this study indicated that the upregulated expression of RIN4 in HLB diseased citrus may aid CLas infection.
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改良植物的过表达硫宁增强了对柑橘溃疡和黄隆（HLB）的抗病性。
影响因子：
5.6
作者：
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