Evaluation of Oxidative Potential of Pyrenequinone Isomers by the Dithiothreitol (DTT) Assay
Evaluation of Oxidative Potential of Pyrenequinone Isomers by the Dithiothreitol (DTT) Assay
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通过二硫苏糖醇 (DTT) 测定评估芘醌异构体的氧化电位
DOI：
10.1080/10406638.2021.1925711
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发表时间：
2022
影响因子：
2.4
通讯作者：
Tohno Susumu
中科院分区：
文献类型：
--
作者：
Okubo Rikito;Kameda Takayuki;Tohno Susumu
大气中的喹类多环芳烃(PAHQ)是颗粒物(PM)中具有氧化还原活性的物质，对人体健康有不良影响。已知几种多环芳烃在二硫苏糖醇(DTT)分析中非常活跃；然而，尚不清楚它们的母体吡喹酮是大气中含量最丰富的多环芳烃(PAHs)之一，是否导致PM提取物中DTT的损失。在这里，我们使用DTT分析方法，评估了三种吡喃酮异构体(4，5-吡喹酮[4，5-PYQ]和1，6-/1，8-吡喹酮[1，6-/1，8-PYQ]的混合物)以及9，10-菲醌(PQN)、1，2-萘醌(1，2-NQ)和1，4-萘醌(1，4-NQ)的氧化电位，其中DTT损失率已被检测过。我们的结果表明，邻位PAHQ对DTT的消耗很快，尤其是4，5-PYQ。4，5-PYQ ∼ PQN>；1，2-NQ>；1，4-NQ∼1，6-/1，8-PYQ。在本研究中，4，5-PYQ首次被证实具有DTT活性。4，5-PYQ的DTT消耗速率为14.6 ± 0.8 / /PAHQ-MOL，与目前DTT测定中活性最高的PQN(14.4± 0.1PAHQ- )相当。
Atmospheric quinoid polycyclic aromatic hydrocarbons (PAHQs) have adverse health effects as redox-active species in particulate matter (PM). Several PAHQs are known to be very reactive in the dithiothreitol (DTT) assay; however, it is unclear if pyrenequinones, their parent pyrene is one of the most abundant polycyclic aromatic hydrocarbons (PAHs) in the atmosphere, contribute to the loss of DTT in PM extracts. Herein, by employing the DTT assay, we evaluated oxidative potentials of three pyrenequinone isomers (4,5-pyrenequinone [4,5-PyQ] and a mixture of 1,6-/1,8-pyrenequinones [1,6-/1,8-PyQ]), along with 9,10-phenanthrenequinone (PQN), 1,2-naphthoquinone (1,2-NQ), and 1,4-naphthoquinone (1,4-NQ), of which DTT loss rates were examined previously. Our results demonstrate that the DTT consumption by ortho-type PAHQs is fast, particularly by 4,5-PyQ. The order of DTT loss rate by the PAHQs tested in this study was as follows: 4,5-PyQ ∼ PQN > 1,2-NQ > 1,4-NQ ∼ 1,6-/1,8-PyQ. 4,5-PyQ was confirmed to be active in the DTT assay for the first time in this study. The DTT consumption rate by 4,5-PyQ is 14.6 ± 0.8 mol/min/PAHQ-mol, which is comparable to that of PQN (14.4 ± 0.1 mol/min/PAHQ-mol) known as the most active PAHQs by the DTT assay so far.
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开发抗氧化剂测定法来研究空气颗粒物的氧化潜力
DOI：
--
发表时间：
2019
期刊：
影响因子：
--
作者：
Pourya Shahpoury;T. Harner;G. Lammel;Steven Lelieveld;Haijie Tong;Jake Wilson
通讯作者：
Jake Wilson
柴油机尾气颗粒正己烷不溶部分的氧化能力和毒性。
影响因子：
3.8
作者：
Hirotoshi Shima;E. Koike;R. Shinohara;Takahiro Kobayashi
通讯作者：
Takahiro Kobayashi
影响因子：
158.5
作者：
Miller, Kristin A.;Siscovick, David S.;Kaufman, Joel D.
通讯作者：
Kaufman, Joel D.
影响因子：
6.3
作者：
Charrier JG;Anastasio C
通讯作者：
Anastasio C