The Exosome Total Isolation Chip.
The Exosome Total Isolation Chip.
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DOI：
10.1021/acsnano.7b04878
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发表时间：
2017-11-28
期刊：
影响因子：
17.1
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Liu F;Vermesh O;Mani V;Ge TJ;Madsen SJ;Sabour A;Hsu EC;Gowrishankar G;Kanada M;Jokerst JV;Sierra RG;Chang E;Lau K;Sridhar K;Bermudez A;Pitteri SJ;Stoyanova T;Sinclair R;Nair VS;Gambhir SS;Demirci U
循环肿瘤来源的细胞外囊泡（EV）已成为一个有前途的来源，用于识别癌症生物标志物，用于早期癌症检测。然而，迄今为止，EV的临床实用性受到以下事实的限制：大多数EV分离方法是繁琐的、非标准化的，并且需要庞大的仪器，例如超离心（UC）。在这里，我们报告了一种基于大小的EV分离工具，称为ExoTIC（exosome total isolation chip），它简单，易于使用，模块化，便于从生物流体中分离出高产量和高纯度的EV。ExoTIC的EV产量比UC高约4-1000倍，并且EV衍生的蛋白质和microRNA水平在两种方法之间具有良好的相关性。此外，我们证明了ExoTIC是一个模块化平台，可以根据大小对癌细胞系EV的异质群体进行分选。此外，我们利用ExoTIC从癌症患者临床样本中分离EV，包括血浆，尿液和灌洗液，证明该设备对癌症和其他疾病的广泛适用性。最后，ExoTIC从小样本量中有效分离EV的能力为小动物肿瘤模型的临床前研究和基于EV的现场临床检测开辟了途径。
Circulating tumor-derived extracellular vesicles (EVs) have emerged as a promising source for identifying cancer biomarkers for early cancer detection. However, the clinical utility of EVs has thus far been limited by the fact that most EV isolation methods are tedious, nonstandardized, and require bulky instrumentation such as ultracentrifugation (UC). Here, we report a size-based EV isolation tool called ExoTIC (exosome total isolation chip), which is simple, easy-to-use, modular, and facilitates high-yield and high-purity EV isolation from biofluids. ExoTIC achieves an EV yield ~4–1000-fold higher than that with UC, and EV-derived protein and microRNA levels are well-correlated between the two methods. Moreover, we demonstrate that ExoTIC is a modular platform that can sort a heterogeneous population of cancer cell line EVs based on size. Further, we utilize ExoTIC to isolate EVs from cancer patient clinical samples, including plasma, urine, and lavage, demonstrating the device’s broad applicability to cancers and other diseases. Finally, the ability of ExoTIC to efficiently isolate EVs from small sample volumes opens up avenues for preclinical studies in small animal tumor models and for point-of-care EV-based clinical testing from fingerprick quantities (10–100 μL) of blood.
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
影响因子：
3.2
作者：
Molino C;Mocerino C;Braucci A;Riccardi F;Trunfio M;Carrillo G;Vitale MG;Cartenì G;De Sena G;Breast Unit Cardarelli Hospital, Naples, Italy
通讯作者：
Breast Unit Cardarelli Hospital, Naples, Italy
影响因子：
3.4
作者：
Kalra, Hina;Adda, Christopher G.;Mathivanan, Suresh
通讯作者：
Mathivanan, Suresh
DOI：
10.1083/jcb.101.3.942
发表时间：
1985-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pan BT;Teng K;Wu C;Adam M;Johnstone RM
通讯作者：
Johnstone RM
DOI：
10.1152/ajprenal.00434.2006
发表时间：
2007-05-01
影响因子：
4.2
作者：
Cheruvanky, Anita;Zhou, Hua;Star, Robert A.
通讯作者：
Star, Robert A.