Rapid prenatal diagnosis and exclusion of epidermolysis bullosa using novel antibody probes.
Rapid prenatal diagnosis and exclusion of epidermolysis bullosa using novel antibody probes.
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使用新型抗体探针快速产前诊断和排除大疱性表皮松解症。
DOI：
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发表时间：
1986
影响因子：
6.5
通讯作者：
Dcparrn
中科院分区：
文献类型：
--
作者：
Adrian;H. M. Hcagcrty;A. R. Kennedy;David;Gunner;A. Robin;Eady;P. F.R.C.;Dcparrn
在妊娠19周时，通过使用单克隆抗体LH 7:2对胎儿皮肤活检样本进行间接免疫荧光检查，成功地实现了隐性营养不良大疱性表皮松解症的产前诊断。胎儿出现明显的水泡，透射电子显微镜（TEM）证实了诊断。在另外3例有致死性大疱性结缔组织表皮松解症危险的妊娠中，使用多克隆抗体AA3排除诊断。在所有这些研究中，结果在收到样品后4小时内可获得。这些新技术为两种严重隐性大疱性表皮松解症的中期产前诊断提供了一种快速简便的替代方法。
Prenatal diagnosis of recessive dystrophic epidermolysis bullosa was successfully achieved at 19 weeks' gestation by indirect immunofluorescence examination of a fetal skin biopsy sample using the monoclonal antibody LH 7:2. The abortus displayed marked blistering and the diagnosis was confirmed by transmission electron microscopy (TEM). In 3 further pregnancies at risk for lethal junctional epidermolysis bullosa the diagnosis was excluded using the polyclonal antibody AA3. In all these studies the results were available within 4 h of receiving the samples. These new techniques offer a quick and simple alternative to TEM for midtrimester prenatal diagnosis of 2 severe recessive forms of epidermolysis bullosa.
使用对苯二胺封固缓冲液增强特异性免疫荧光结果。
DOI：
10.1111/1523-1747.ep12508101
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Huff,JC;Weston,WL;Wanda,KD
通讯作者：
Wanda,KD