The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress.
The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress.
复制标题
DOI：
10.1093/jxb/err039
复制
发表时间：
2011-07
影响因子：
6.9
通讯作者：
Martinec J
中科院分区：
文献类型：
--
作者：
Kocourková D;Krcková Z;Pejchar P;Veselková S;Valentová O;Wimalasekera R;Scherer GF;Martinec J
磷脂酰胆碱水解型磷脂酶C，又称非特异性磷脂酶C(NPC)，是植物磷脂酶家族的新成员，对缺磷、铝毒等环境胁迫作出反应，在根发育和油菜素内酯信号转导中起重要作用。NPC4基因是拟南芥的6个NPC基因之一，在盐胁迫下高度诱导表达。在盐处理后3 h至6 h达到最大值，并与盐浓度有关。PNPC4：GUS植株的组织化学分析结果表明，盐诱导的表达定位于根尖。在生化水平上，早在盐处理30 m in后，即可观察到以二甘油积累为标志的鼻咽癌酶活性升高。NPC4基因敲除植株的表型分析表明，与野生型植株相比，NPC4基因敲除植株对盐分的敏感性增加。盐胁迫下，npc4植株根系较短，鲜重较低，种子发芽率较低。在盐处理的npc4植株中，脱落酸相关基因ABI1、ABI2、Rab18、PP2CA和SOT12的表达水平显著降低。这些观察结果表明，NPC4在拟南芥对盐胁迫的反应中发挥了作用。
Phosphatidylcholine-hydrolysing phospholipase C, also known as non-specific phospholipase C (NPC), is a new member of the plant phospholipase family that reacts to environmental stresses such as phosphate deficiency and aluminium toxicity, and has a role in root development and brassinolide signalling. Expression of NPC4, one of the six NPC genes in Arabidopsis, was highly induced by NaCl. Maximum expression was observed from 3 h to 6 h after the salt treatment and was dependent on salt concentration. Results of histochemical analysis of PNPC4:GUS plants showed the localization of salt-induced expression in root tips. On the biochemical level, increased NPC enzyme activity, indicated by accumulation of diacylglycerol, was observed as early as after 30 min of salt treatment of Arabidopsis seedlings. Phenotype analysis of NPC4 knockout plants showed increased sensitivity to salinity as compared with wild-type plants. Under salt stress npc4 plants had shorter roots, lower fresh weight, and reduced seed germination. Expression levels of abscisic acid-related genes ABI1, ABI2, RAB18, PP2CA, and SOT12 were substantially reduced in salt-treated npc4 plants. These observations demonstrate a role for NPC4 in the response of Arabidopsis to salt stress.
登录
查看更多内容
影响因子：
56.9
作者：
Fang, YM;Vilella-Bach, M;Chen, J
通讯作者：
Chen, J
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.2
作者：
Gaude, Nicole;Nakamura, Yuki;Doermann, Peter
通讯作者：
Doermann, Peter
影响因子：
7.2
作者：
Katagiri, T;Ishiyama, K;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
27.5
作者：
Hong, Yueyun;Zheng, Suqin;Wang, Xuemin
通讯作者：
Wang, Xuemin