Overexpression of the WAPO-A1 gene increases the number of spikelets per spike in bread wheat
Overexpression of the WAPO-A1 gene increases the number of spikelets per spike in bread wheat
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WAPO-A1 基因的过度表达增加了面包小麦中每个穗的小穗数量
DOI：
10.1101/2022.04.01.486512
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wittern L
中科院分区：
文献类型：
--
作者：
Wittern L
利用两个小麦魔术群体，在7AL和7BL染色体上定位了两个控制每穗颖花数的同源QTL。建立了一组与7AL上的QTL进行对比的品系，为7AL QTL的验证和精细定位以及先前描述的候选基因小麦APO1的同源基因(WAPO1)的鉴定奠定了基础。利用在低SPS和高SPS品系中的转基因过表达，我们为该基因在决定六倍体小麦SPS中的作用提供了功能验证。我们发现该基因的表达水平与田间条件下的多个魔方品系以及温室种植的转基因品系中的SPS呈正相关。这项工作突出了WAPO1在六倍体小麦中进一步增产的潜在用途。WAPO1和SPS对产量的影响取决于其他遗传和环境因素，因此，需要一个微妙平衡的表达水平，以避免产生有害的多效性表型。
Two homoeologous QTLs for number of spikelets per spike (SPS) were mapped on chromosomes 7AL and 7BL using two wheat MAGIC populations. Sets of lines contrasting for the QTL on 7AL were developed which allowed for the validation and fine mapping of the 7AL QTL and for the identification of a previously described candidate gene,WHEAT ORTHOLOG OF APO1(WAPO1). Using transgenic overexpression in both a low and a high SPS line, we provide a functional validation for the role of this gene in determining SPS also in hexaploid wheat. We show that the expression levels of this gene positively correlate with SPS in multiple MAGIC founder lines under field conditions as well as in transgenic lines grown in the greenhouse. This work highlights the potential use ofWAPO1in hexaploid wheat for further yield increases. The impact ofWAPO1and SPS on yield depends on other genetic and environmental factors, hence, will require a finely balanced expression level to avoid the development of detrimental pleiotropic phenotypes.
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影响因子：
3.3
作者：
Mathew B;Léon J;Sannemann W;Sillanpää MJ
通讯作者：
Sillanpää MJ
影响因子：
7.2
作者：
Ikeda, Kyoko;Ito, Momoyo;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
早熟本身×温度相互作用：对小麦叶片、小穗和小花发育的影响
影响因子：
6.9
作者：
P. Prieto;H. Ochagavía;S. Griffiths;G. Slafer
通讯作者：
G. Slafer
影响因子：
6.9
作者：
Shaw LM;Lyu B;Turner R;Li C;Chen F;Han X;Fu D;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
13.8
作者：
Wang S;Wong D;Forrest K;Allen A;Chao S;Huang BE;Maccaferri M;Salvi S;Milner SG;Cattivelli L;Mastrangelo AM;Whan A;Stephen S;Barker G;Wieseke R;Plieske J;International Wheat Genome Sequencing Consortium;Lillemo M;Mather D;Appels R;Dolferus R;Brown-Guedira G;Korol A;Akhunova AR;Feuillet C;Salse J;Morgante M;Pozniak C;Luo MC;Dvorak J;Morell M;Dubcovsky J;Ganal M;Tuberosa R;Lawley C;Mikoulitch I;Cavanagh C;Edwards KJ;Hayden M;Akhunov E
通讯作者：
Akhunov E