Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans.
Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans.
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DOI：
10.1038/s41598-021-84516-y
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发表时间：
2021-03-03
影响因子：
4.6
通讯作者：
Fang-Yen C
中科院分区：
文献类型：
--
作者：
Fouad AD;Liu A;Du A;Bhirgoo PD;Fang-Yen C
激光显微手术一直是评估特定细胞和组织功能的重要手段。大多数激光消融系统使用短的、高度聚焦的激光脉冲来产生等离子体介导的病变，其尺寸在光波长的数量级上。虽然病灶的小尺寸可以实现高空间分辨率的消融，但它也使得消融更大的结构变得困难。我们开发了一种红外激光烧蚀系统，该系统能够通过激光脉冲的持续时间和幅度来调节光斑的大小。我们在线虫中使用激光系统来杀死单个神经元，切断背侧和腹侧神经索，这些结构很难用等离子消融系统损伤。我们使用这些消融来研究乙酰胆碱受体ACR-2的功能获得突变体的惊厥来源。切断腹侧神经索可使惊厥分别发生在病变前方和后方，提示惊厥可由运动回路的不同亚群独立发生。
Laser microsurgery has long been an important means of assessing the functions of specific cells and tissues. Most laser ablation systems use short, highly focused laser pulses to create plasma-mediated lesions with dimensions on the order of the wavelength of light. While the small size of the lesion enables ablation with high spatial resolution, it also makes it difficult to ablate larger structures. We developed an infrared laser ablation system capable of thermally lesioning tissues with spot sizes tunable by the duration and amplitude of laser pulses. We used our laser system in the roundworm C. elegans to kill single neurons and to sever the dorsal and ventral nerve cords, structures that are difficult to lesion using a plasma-based ablation system. We used these ablations to investigate the source of convulsions in a gain-of-function mutant for the acetylcholine receptor ACR-2. Severing the ventral nerve cord caused convulsions to occur independently anterior and posterior to the lesion, suggesting that convulsions can arise independently from distinct subsets of the motor circuit.
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红外激光诱导的基因表达，用于跟踪单一秀丽隐杆线虫胚胎神经元的发育和功能。
影响因子：
16.6
作者：
Singhal A;Shaham S
通讯作者：
Shaham S
影响因子：
--
作者：
Fang-Yen, Christopher;Gabel, Christopher V.;Samuel, Aravinthan D. T.;Bargmann, Cornelia I.;Avery, Leon
通讯作者：
Avery, Leon
影响因子：
3.7
作者：
Kim E;Sun L;Gabel CV;Fang-Yen C
通讯作者：
Fang-Yen C
影响因子：
5.3
作者：
Gabel, Christopher V.;Gabel, Harrison;Samuel, Aravinthan D. T.
通讯作者：
Samuel, Aravinthan D. T.
影响因子：
3
作者：
Olivier, A
通讯作者：
Olivier, A