Eccentric rheometry for viscoelastic characterization of small, soft, anisotropic, and irregularly shaped biopolymer gels and tissue biopsies.
Eccentric rheometry for viscoelastic characterization of small, soft, anisotropic, and irregularly shaped biopolymer gels and tissue biopsies.
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DOI：
10.1007/s10439-012-0532-5
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发表时间：
2012-08
影响因子：
3.8
通讯作者：
Billiar, Kristen L.
中科院分区：
文献类型：
--
作者：
Cirka, Heather A.;Koehler, Stephan A.;Farr, William W.;Billiar, Kristen L.
组织活检和细胞重塑水凝胶的物理性质的量化对于了解组织发育和病理生理组织重塑至关重要。然而，由于这些材料样品的模数低、尺寸小、形状不规则和各向异性，使用标准流变学方法准确地表征粘弹性是有问题的。这项工作的目标是利用图像分析将旋转流变仪扩展到这些样品。在这种方法中，样品被偏置以增加产生的扭矩；使用定制的透明玻璃几何结构、直角棱镜和照相机来确定样品相对于旋转轴的准确形状和位置，以计算样品的剪切模数G‘。在中心构型和偏心构型下测试的标准聚二甲基硅氧烷凝胶的G‘值无统计学差异(分别为137±37 kPa和126±8 kpa，p=0.58)，表明该方法是准确的。此外，圆形和不规则形状的胶原凝胶的G‘值也相同(31±1.8pA和31±5.1pA，p=0.29)。用该方法成功地检测了从病人身上回收的血块和脂斑(G‘~4kpa)，证明了该方法在临床诊断中的适用性。
Quantification of the physical properties of tissue biopsies and cell-remodeled hydrogels is critical for understanding tissue development and pathophysiological tissue remodeling. However, due to the low modulus, small size, irregular shape, and anisotropy of samples from these materials, accurate viscoelastic characterization using standard rheometric methods is problematic. The goal of this work is to utilize image analysis to extend rotational rheometry to these samples. In this method, the sample is offset to increase the torque generated; a custom clear glass geometry, right angle prism, and camera are used to determine the exact shape and location of the sample relative to the axis of rotation for calculation of the sample shear modulus, G’. Values of G’ for standard polydimethylsiloxane gels tested in centered and eccentric configurations were not statistically different (respecitvely 137 ± 37 kPa and 126 ± 8 kPa, p = 0.58), indicating accuracy of the method. Additionally, G’ values from circular and irregularly shaped collagen gels yielded equivalent results (31 ± 1.8 Pa and 31 ± 5.1 Pa, p = 0.29). A blood clot and a lipid plaque sample recovered from human patients (G’ ~ 4 kPa) were successfully tested with this method, demonstrating applicability to clinical diagnostics.
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人皮肤对低生理相关剪切的粘弹性反应。
影响因子：
2.4
作者：
Holt, Brian;Tripathi, Anubhav;Morgan, Jeffrey
通讯作者：
Morgan, Jeffrey
DOI：
10.1021/jp807749f
发表时间：
2009-03-26
期刊：
The journal of physical chemistry. B
影响因子：
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作者：
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MacKintosh FC
影响因子：
2.3
作者：
Nasseri, S;Bilston, LE;Phan-Thien, N
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Phan-Thien, N
影响因子：
4.8
作者：
Leung, Lester Y.;Tian, David;Tschumperlin, Daniel J.
通讯作者：
Tschumperlin, Daniel J.
DOI：
10.1115/1.1449904
发表时间：
2002-04-01
影响因子：
1.7
作者：
Roeder, BA;Kokini, K;Voytik-Harbin, SL
通讯作者：
Voytik-Harbin, SL