Morphological study by an ‘in vivo cryotechnique’ of the shape of erythrocytes circulating in large blood vessels
Morphological study by an ‘in vivo cryotechnique’ of the shape of erythrocytes circulating in large blood vessels
复制标题
通过“体内冷冻技术”对大血管中循环的红细胞形状进行形态学研究
作者：
M. Xue;Y. Kato;N. Terada;Y. Fujii;T. Baba;S. Ohno
用我们的“体内冷冻技术”研究了小鼠大血管中循环红细胞形状的变化。用预冷刀垂直切割腹主动脉和下腔静脉（IVC），同时将异戊烷-丙烷混合物（-193 °C）倒在其上冷冻。将其在含有2%四氧化锇的丙酮中冷冻置换。将一些标本包埋在Quetol-812中，并通过光学或透射电子显微镜检查厚切片或切片。使用连续切片重建天然红细胞的三维图像。将其他样品转移至叔丁醇中并冷冻干燥，用于扫描电子显微镜检查。组织表面被充分冷冻以防止大冰晶形成，并且红细胞形状也被保留。循环红细胞的形状在腹主动脉中出现变化，但很少观察到典型的双凹盘状。相反，IVC中的红细胞呈近似双凹面盘状。我们的体内冷冻技术是有用的澄清在大血管中循环的红细胞的体内形态。
Changes in the shape of erythrocytes circulating in large blood vessels of mice were examined by our ‘in vivo cryotechnique’. The abdominal aorta and inferior vena cava (IVC) were cut vertically with a precooled knife and simultaneously an isopentane–propane mixture (−193°C) was poured over them for freezing. They were freeze‐substituted in acetone containing 2% osmium tetroxide. Some specimens were embedded in Quetol‐812, and thick or ultrathin sections were examined by light or transmission electron microscopy. Serial ultrathin sections were used to reconstruct 3‐dimensional images of native erythrocytes. Others were transferred into t‐butyl alcohol and freeze‐dried for scanning electron microscopy. The tissue surfaces were sufficiently frozen to prevent large ice crystal formation, and erythrocyte shapes were also preserved. The shapes of circulating erythrocytes appeared to be varied in the abdominal aorta but typical biconcave discoid shapes were rarely observed. Conversely, erythrocytes were approximately biconcave discoid in shape in the IVC. Our in vivo cryotechnique was useful for clarifying the in vivo morphology of erythrocytes circulating in large blood vessels.
影响因子：
3.6
作者：
N. Mohandas;J. Chasis
通讯作者：
N. Mohandas;J. Chasis
红细胞膜永久变形对血影蛋白二聚体-四聚体平衡的依赖性：对不可逆镰状细胞永久膜变形的影响。
影响因子：
20.3
作者：
Liu,SC;Derick,LH;Palek,J
通讯作者：
Palek,J