Expression analysis of immune related genes identified from the coelomocytes of sea cucumber (Apostichopus japonicus) in response to LPS challenge.
Expression analysis of immune related genes identified from the coelomocytes of sea cucumber (Apostichopus japonicus) in response to LPS challenge.
复制标题
从海参 (Apostichopus japonicus) 体腔细胞中鉴定出的免疫相关基因响应 LPS 攻击的表达分析。
DOI：
10.3390/ijms151119472
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发表时间：
2014-10-27
影响因子：
5.6
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Dong Y;Sun H;Zhou Z;Yang A;Chen Z;Guan X;Gao S;Wang B;Jiang B;Jiang J
仿刺参（Apostichopus）在后口动物的进化过程中处于基础地位，也是重要的水产养殖物种。为了鉴定更多的免疫效应物，对A.使用Illumina HiSeq™ 2000平台进行响应于脂多糖（LPS）攻击的巨噬体腔细胞。共筛选出107个差异表达基因，分为病原体识别（25个基因）、细胞骨架重组（27个基因）、炎症（41个基因）和凋亡（14个基因）4类。分析它们以阐明宿主-病原体相互作用和下游信号转导的机制。10个代表性基因的实时定量聚合酶链反应（qRT-PCR）验证了RNA测序结果的准确性和可靠性，相关系数为0.88至0.98，p值<0.05。对免疫相关基因表达的分析将有助于了解A.抗病原菌入侵和抗性标记筛选策略的研究。
The sea cucumber (Apostichopus japonicus) occupies a basal position during the evolution of deuterostomes and is also an important aquaculture species. In order to identify more immune effectors, transcriptome sequencing of A. japonicus coelomocytes in response to lipopolysaccharide (LPS) challenge was performed using the Illumina HiSeq™ 2000 platform. One hundred and seven differentially expressed genes were selected and divided into four functional categories including pathogen recognition (25 genes), reorganization of cytoskeleton (27 genes), inflammation (41 genes) and apoptosis (14 genes). They were analyzed to elucidate the mechanisms of host-pathogen interactions and downstream signaling transduction. Quantitative real-time polymerase chain reactions (qRT-PCRs) of 10 representative genes validated the accuracy and reliability of RNA sequencing results with the correlation coefficients from 0.88 to 0.98 and p-value <0.05. Expression analysis of immune-related genes after LPS challenge will be useful in understanding the immune response mechanisms of A. japonicus against pathogen invasion and developing strategies for resistant markers selection.
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影响因子：
4.7
作者：
Kingham, PJ;Pocock, JM
通讯作者：
Pocock, JM
影响因子：
30.3
作者：
Haenisch, Jan;Koelm, Robert;Stradal, Theresia E. B.
通讯作者：
Stradal, Theresia E. B.
DOI：
10.1083/jcb.200210053
发表时间：
2003-02-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hillier BJ;Vacquier VD
通讯作者：
Vacquier VD
影响因子：
4.3
作者：
Bulgakov, Aleksandr A.;Eliseikina, Marina G.;Rasskazov, Valery A.
通讯作者：
Rasskazov, Valery A.
影响因子：
2.8
作者：
Holm, Kristina;Dupont, Samuel;Hernroth, Bodil
通讯作者：
Hernroth, Bodil