Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas.
Direct electrochemical measurement of metanephrines in spot urine samples for the diagnosis of phaeochromocytomas.
复制标题
直接电化学测量点尿样本中的变肾上腺素用于诊断嗜铬细胞瘤
DOI：
10.1038/s41598-017-08612-8
复制
发表时间：
2017-08-14
影响因子：
4.6
通讯作者：
Ding M
中科院分区：
文献类型：
--
作者：
Shi ZH;Zhang XQ;Zhen QN;Zuo M;Tian G;He YF;Ding M
甲肾上腺素（MN）被认为是诊断嗜铬细胞瘤（PHEO）的一线生化指标。在这项研究中，我们建立了一个简单的电化学方法定量测定现场尿样中的MN。由于MN含有羟基苯基，它们可以在一定的电位下被氧化成奎宁，这可以通过使用未修饰的丝网印刷碳电极（SPCE）的差分脉冲伏安法（DPV）进一步检测。同时采用固相萃取技术消除样品中的基体效应。因此，在0.25 mg/L至12.5 mg/L的线性范围内筛选来自提取的尿样的游离MN。MN的最低定量限（LLOQ）为0.25 mg/L，检测限（LOD）为0.05 mg/L。精密度和回收率均满足临床应用要求。用该方法分析了22例PHEO患者和63例对照组的尿样。ROC曲线下面积为0.981（95%CI，0.958-1.000），以0.404 mg/L为界值，敏感性为95.5%，特异性为92.4%。总的来说，所提出的方法提供了一个具有成本效益的，快速和简单的工具，用于临床诊断PHEO。
Metanephrines (MNs) were suggested as a potential first-line biochemical index for the diagnosis of phaeochromocytomas (PHEO). In this study, we developed a simple electrochemical method for the quantitative measurement of MNs in spot urine samples. As MNs contain a hydroxyphenyl group, they could be oxidized at a certain potential to quinines, which could be further detected by the differential pulse voltammetry (DPV) method using unmodified screen-printed carbon electrode (SPCE). Meanwhile, the solid phase extraction (SPE) technique was used to eliminate the matrix effect in the samples. Consequently, free MNs from the extracted urine sample were screened in a linear range from 0.25 mg/L to 12.5 mg/L. The lowest limit of quantification (LLOQ) for MNs was 0.25 mg/L and the limit of detection (LOD) was 0.05 mg/L. Both the precisions and recoveries were sufficient for clinical applications. The urine samples from 22 patients with PHEO and 63 controls were analyzed by the proposed method. The area under the ROC curve was 0.981 (95% CI, 0.958–1.000) with the sensitivity of 95.5% and the specificity of 92.4% at the cut-off value of 0.404 mg/L in these urine samples. Overall, the proposed method provides a cost-effective, rapid and simple tool for clinical diagnosis of PHEO.
登录
查看更多内容
配备石墨烯/聚苯胺/金纳米粒子/葡萄糖氧化酶生物复合材料修饰丝网印刷电极的纸盘：迈向全血糖测定
影响因子：
12.6
作者：
Kong, Fen-Ying;Gu, Sai-Xi;Wang, Wei
通讯作者：
Wang, Wei
影响因子：
6.8
作者：
Mullins, Fiona;O'Shea, Paula;Tormey, William
通讯作者：
Tormey, William
影响因子：
6.8
作者：
Pussard, Eric;Chaouch, Amel;Said, Toihiri
通讯作者：
Said, Toihiri
DOI：
10.1016/j.jchromb.2011.10.005
发表时间：
2011-12-01
影响因子：
3
作者：
Clark, Zlatuse D.;Frank, Elizabeth L.
通讯作者：
Frank, Elizabeth L.
影响因子：
5.8
作者：
Lenders, Jacques W. M.;Duh, Quan-Yang;Young, William F., Jr.
通讯作者：
Young, William F., Jr.