Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in the ygl-1 Mapping Interval with Recombination Suppression.
Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in the ygl-1 Mapping Interval with Recombination Suppression.
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甘蓝黄绿叶突变体在ygl-1作图区间与重组抑制相关的差异表达基因
DOI：
10.3390/ijms19102936
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发表时间：
2018-09-27
影响因子：
5.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Liu X;Yu H;Han F;Li Z;Fang Z;Yang L;Zhuang M;Lv H;Liu Y;Li Z;Li X;Zhang Y
虽然甘蓝黄绿叶突变体YL-1的遗传学和初步定位已被广泛研究，但尚未发现与YL-1的黄绿叶突变体相关的转录组分析。两个群体的定位结果表明，黄绿叶基因ygl-1位于重组抑制基因组区域。然后，使用批量分离RNA-seq（BSR）来鉴定F3群体（YL-1 × 11-192）和BC 2群体（YL-1 × 01-20）的差异表达基因（DEG）。在37，286个独特基因中，在来自F3和BC 2群体的黄叶和正常叶池之间检测到5730和4118个DEG。四池的BSR分析大大减少了常见的DEG的数量从4924到1112。在ygl-1基因定位区域，共鉴定出43个共同的DEG。其中5个DEG与叶绿体相关，包括下调的Bo 1g 087310、Bo 1g 094360和Bo 1g 098630以及上调的Bo 1g 059170和Bo 1g 098440。通过qRT-PCR排除Bo 1g 098440和Bo 1g 098630基因。因此，我们推断，这三个DEG（Bo 1g 094360，Bo 1g 087310，和Bo 1g 059170）在作图区间可能与黄绿叶突变表型的发展密切相关。
Although the genetics and preliminary mapping of the cabbage yellow-green-leaf mutant YL-1 has been extensively studied, transcriptome profiling associated with the yellow-green-leaf mutant of YL-1 has not been discovered. Positional mapping with two populations showed that the yellow-green-leaf gene ygl-1 is located in a recombination-suppressed genomic region. Then, a bulk segregant RNA-seq (BSR) was applied to identify differentially expressed genes (DEGs) using an F3 population (YL-1 × 11-192) and a BC2 population (YL-1 × 01-20). Among the 37,286 unique genes, 5730 and 4118 DEGs were detected between the yellow-leaf and normal-leaf pools from the F3 and BC2 populations. BSR analysis with four pools greatly reduced the number of common DEGs from 4924 to 1112. In the ygl-1 gene mapping region with suppressed recombination, 43 common DEGs were identified. Five of the DEGs were related to chloroplasts, including the down-regulated Bo1g087310, Bo1g094360, and Bo1g098630 and the up-regulated Bo1g059170 and Bo1g098440. The Bo1g098440 and Bo1g098630 genes were excluded by qRT-PCR. Hence, we inferred that these three DEGs (Bo1g094360, Bo1g087310, and Bo1g059170) in the mapping interval may be tightly associated with the development of the yellow-green-leaf mutant phenotype.
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影响因子：
11.6
作者：
Carol, P;Stevenson, D;Kuntz, M
通讯作者：
Kuntz, M
影响因子：
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