Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis.
Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis.
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DOI：
10.1016/j.clim.2008.09.016
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Kohno S
中科院分区：
文献类型：
--
作者：
Yoshida A;Isomoto H;Hisatsune J;Nakayama M;Nakashima Y;Matsushima K;Mizuta Y;Hayashi T;Yamaoka Y;Azuma T;Moss J;Hirayama T;Kohno S
CC 趋化因子配体 20 (CCL20) 吸引表达 CC 趋化因子受体 6 (CCR6) 的细胞。使用取自 42 名感染幽门螺杆菌的受试者和 42 名未感染受试者的胃窦的内窥镜活检，分别通过实时聚合酶链反应和酶联免疫吸附测定来测量粘膜 CCL20 mRNA 和蛋白质水平。还测量了 CCL19 mRNA 和蛋白质水平，以及 CCL21 mRNA 水平。幽门螺杆菌阳性患者的 CCL20 mRNA 和蛋白水平显着升高，并在成功根除后显着下降。 CCL19 和 CCL21 表达水平在幽门螺杆菌感染组和未感染组中相当。 CCL20浓度与慢性胃炎的程度相关。免疫组织化学和体外感染实验表明CCL20主要由胃上皮产生。表达 CCR6 的细胞，包括 CD45RO+ 记忆 T 淋巴细胞和肌成束蛋白+-CD1a+ 未成熟树突细胞，浸润到表达 CCL20 的上皮细胞附近。 CCL20/CCR6 相互作用可能参与幽门螺杆菌相关胃炎的发生。
CC chemokine ligand 20 (CCL20) attracts CC chemokine receptor 6 (CCR6)-expressing cells. Using endoscopic biopsies taken from the gastric antrum of 42 subjects infected with H. pylori and 42 uninfected subjects, mucosal CCL20 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. CCL19 mRNA and protein levels, as well as CCL21 mRNA levels, were also measured. The CCL20 mRNA and protein levels were significantly elevated in H. pylori-positive patients and substantially decreased after successful eradication. CCL19 and CCL21 expression levels were comparable in the H. pylori-infected and the uninfected groups. The CCL20 concentrations correlated with the degree of chronic gastritis. Immunohistochemistry and the in vitro infection assay showed that CCL20 was principally produced by the gastric epithelium. CCR6-expressing cells, including CD45RO+ memory T lymphocytes and fascin+-CD1a+ immature dendritic cells, infiltrated close to the CCL20-expressing epithelial cells. The CCL20/CCR6 interaction may be involved in the development of H. pylori-associated gastritis.
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影响因子：
3.1
作者：
Wu, Yi-Ying;Tsai, Hwei-Fang;Hsu, Ping-Ning
通讯作者：
Hsu, Ping-Ning
影响因子：
24.5
作者：
Middel, P;Raddatz, D;Radzun, HJ
通讯作者：
Radzun, HJ
影响因子：
15.3
作者：
Dieu, M C;Vanbervliet, B;Vicari, A;Bridon, J M;Oldham, E;Ait-Yahia, S;Briere, F;Zlotnik, A;Lebecque, S;Caux, C
通讯作者：
Caux, C
影响因子：
9.8
作者：
Isomoto, H;Ueno, H;Kohno, S
通讯作者：
Kohno, S
影响因子：
64.8
作者：
Baggiolini, M
通讯作者：
Baggiolini, M