In vitro desensitization of human skin mast cells.
In vitro desensitization of human skin mast cells.
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DOI：
10.1007/s10875-011-9605-8
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发表时间：
2012-02
影响因子：
9.1
通讯作者：
Schwartz LB
中科院分区：
文献类型：
--
作者：
Zhao W;Gomez G;Macey M;Kepley CL;Schwartz LB
Desensitization is a clinical procedure whereby incremental doses of a drug are administered over several hours to a sensitive patient until a therapeutic dose and clinical tolerance are achieved. Clinical tolerance may occur in part by attenuating the mast cell response. In the present study, primary human skin mast cells were used to establish and characterize an in vitro model of desensitization. Mast cells in culture were armed with allergen-specific (4-hydroxy-3-nitro-phenylacety and Der p2) and non-specific IgE antibodies, and then desensitized by incremental exposures to 4-hydroxy-3-nitrophenylacety-BSA. This desensitization procedure abrogated the subsequent degranulation response to the desensitizing allergen, to an unrelated allergen, and to IgG anti-FcεRI, but not to C5a, substance P, compound 48/80, and calcium ionophore. Desensitized cells regained their FcεRI-dependent degranulation capability by 24–48 h after free allergen had been removed. Therefore, sensitized human skin mast cells are reversibly desensitized in vitro by exposure to incremental doses of that allergen, which also cross-desensitizes them to an unrelated allergen.
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影响因子：
2.8
作者：
Komiya, A;Hirai, K;Yamaguchi, M
通讯作者：
Yamaguchi, M
影响因子：
158.5
作者：
Brightling, CE;Bradding, P;Pavord, ID
通讯作者：
Pavord, ID
影响因子：
14.2
作者：
STARK, BJ;EARL, HS;SULLIVAN, TJ
通讯作者：
SULLIVAN, TJ
DOI：
10.4049/jimmunol.181.10.7263
发表时间：
2008-11-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Zhao W;Gomez G;Yu SH;Ryan JJ;Schwartz LB
通讯作者：
Schwartz LB
影响因子：
14.2
作者：
Kepley, CL;Youssef, L;Oliver, JM
通讯作者：
Oliver, JM