Electrophysiological characterization of spinal neuron sensitization by elevated calcium channel alpha-2-delta-1 subunit protein.
Electrophysiological characterization of spinal neuron sensitization by elevated calcium channel alpha-2-delta-1 subunit protein.
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DOI：
10.1002/j.1532-2149.2013.00416.x
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发表时间：
2014-05
影响因子：
3.6
通讯作者：
Luo, Z. D.
中科院分区：
文献类型：
--
作者：
Zhou, C.;Luo, Z. D.
Voltage-gated calcium channel α2δ1 subunit is the binding site for gabapentin, an effective drug in controlling neuropathic pain states including thermal hyperalgesia. Hyperalgesia to noxious thermal stimuli in both spinal-nerve-ligated (SNL) and voltage-gated calcium channel α2δ1 over-expressing transgenic (Tg) mice correlates with higher α2δ1 levels in dorsal root ganglia and dorsal spinal cord. In this study, we investigated whether abnormal synaptic transmission is responsible for thermal hyperalgesia induced by elevated α2δ1 expression in these models. Behavioral sensitivities to thermal stimuli were test in L4 SNL and sham mice, as well as in α2δ1 Tg and wild-type mice. Miniature excitatory (mEPSC) and inhibitory (mIPSC) postsynaptic currents were recorded in superficial dorsal spinal cord neurons from these models using whole-cell patch clamp slice recording techniques. The frequency, but not amplitude, of mEPSC in superficial dorsal horn neurons was increased in SNL and α2δ1 Tg mice, which could be attenuated by gabapentin dose dependently. Intrathecal α2δ1 antisense oligodeoxynucleotide treatment diminished increased mEPSC frequency and gabapentin's inhibitory effects in elevated mEPSC frequency in the SNL mice. In contrast, neither the frequency, nor the amplitude, of mIPSC was altered in superficial dorsal horn neurons from the SNL and α2δ1 Tg mice. Our findings support a role of peripheral nerve injury-induced α2δ1 in enhancing presynaptic excitatory input onto superficial dorsal spinal cord neurons that contributes to nociception development.
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影响因子：
5.7
作者：
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通讯作者：
Chung, JM
影响因子：
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DOI：
10.1016/s1098-7339(06)80010-6
发表时间：
1997-05-01
期刊：
REGIONAL ANESTHESIA
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通讯作者：
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通讯作者：
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