Nerve Growth Factor-induced Glutamate Release Is via p75 Receptor, Ceramide, and Ca2+ from Ryanodine Receptor in Developing Cerebellar Neurons*
Nerve Growth Factor-induced Glutamate Release Is via p75 Receptor, Ceramide, and Ca2+ from Ryanodine Receptor in Developing Cerebellar Neurons*
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神经生长因子诱导的谷氨酸释放是通过小脑神经元发育过程中的 p75 受体、神经酰胺和 Ryanodine 受体中的 Ca2 实现的*
DOI：
10.1074/jbc.m304409200
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发表时间：
2003
影响因子：
4.8
通讯作者：
T. Taguchi
中科院分区：
文献类型：
--
作者：
T. Numakawa;H. Nakayama;Shingo Suzuki;T. Kubo;F. Nara;Yumiko Numakawa;D. Yokomaku;T. Araki;T. Ishimoto;A. Ogura;T. Taguchi
Very little is known about the contribution of a low affinity neurotrophin receptor, p75, to neurotransmitter release. Here we show that nerve growth factor (NGF) induced a rapid release of glutamate and an increase of Ca2+ in cerebellar neurons through a p75-dependent pathway. The NGF-induced release occurred even in the presence of the Trk inhibitor K252a. The release caused by NGF but not brain-derived neurotrophic factor was enhanced in neurons overexpressing p75. Further, after transfection of p75-small interfering RNA, which down-regulated the endogenous p75 expression, the NGF-induced release was inhibited, suggesting that the NGF-induced glutamate release was through p75. We found that the NGF-increased Ca2+ was derived from the ryanodine-sensitive Ca2+ receptor and that the NGF-increased Ca2+ was essential for the NGF-induced glutamate release. Furthermore, scyphostatin, a sphingomyelinase inhibitor, blocked the NGF-dependent Ca2+ increase and glutamate release, suggesting that a ceramide produced by sphingomyelinase was required for the NGF-stimulated Ca2+ increase and glutamate release. This action of NGF only occurred in developing neurons whereas the brain-derived neurotrophic factor-mediated Ca2+ increase and glutamate release was observed at the mature neuronal stage. Thus, we demonstrate that NGF-mediated neurotransmitter release via the p75-dependent pathway has an important role in developing neurons.
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DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandler,CE;Parsons,LM;Hosang,M;Shooter,EM
通讯作者：
Shooter,EM
影响因子：
--
作者：
D. Perry;A. Bielawska;Y. Hannun
通讯作者：
D. Perry;A. Bielawska;Y. Hannun
影响因子：
56.9
作者：
DOBROWSKY, RT;WERNER, MH;HANNUN, YA
通讯作者：
HANNUN, YA
生物活性脂质、烷基酰基甘油和神经酰胺的变化发生在感染 HIV 的细胞中。
DOI：
10.1016/s0006-291x(05)81480-9
发表时间：
1992
影响因子：
3.1
作者：
VanVeldhoven,PP;Matthews,TJ;Bolognesi,DP;Bell,RM
通讯作者：
Bell,RM