KinasePhos 2.0: a web server for identifying protein kinase-specific phosphorylation sites based on sequences and coupling patterns.
KinasePhos 2.0: a web server for identifying protein kinase-specific phosphorylation sites based on sequences and coupling patterns.
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DOI：
10.1093/nar/gkm322
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发表时间：
2007-07
影响因子：
14.9
通讯作者：
Hwang JK
中科院分区：
文献类型：
--
作者：
Wong YH;Lee TY;Liang HK;Huang CM;Wang TY;Yang YH;Chu CH;Huang HD;Ko MT;Hwang JK
Due to the importance of protein phosphorylation in cellular control, many researches are undertaken to predict the kinase-specific phosphorylation sites. Referred to our previous work, KinasePhos 1.0, incorporated profile hidden Markov model (HMM) with flanking residues of the kinase-specific phosphorylation sites. Herein, a new web server, KinasePhos 2.0, incorporates support vector machines (SVM) with the protein sequence profile and protein coupling pattern, which is a novel feature used for identifying phosphorylation sites. The coupling pattern [XdZ] denotes the amino acid coupling-pattern of amino acid types X and Z that are separated by d amino acids. The differences or quotients of coupling strength CXdZ between the positive set of phosphorylation sites and the background set of whole protein sequences from Swiss-Prot are computed to determine the number of coupling patterns for training SVM models. After the evaluation based on k-fold cross-validation and Jackknife cross-validation, the average predictive accuracy of phosphorylated serine, threonine, tyrosine and histidine are 90, 93, 88 and 93%, respectively. KinasePhos 2.0 performs better than other tools previously developed. The proposed web server is freely available at http://KinasePhos2.mbc.nctu.edu.tw/.
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影响因子：
3
作者：
Huang, HD;Lee, TY;Huang, KT
通讯作者：
Huang, KT
影响因子：
2.9
作者：
Lin, HH;Han, LY;Chen, YZ
通讯作者：
Chen, YZ
影响因子：
2.9
作者：
Liang, HK;Huang, CM;Hwang, JK
通讯作者：
Hwang, JK
Kinasephos：用于识别蛋白激酶特异性磷酸化位点的Web工具。
影响因子：
14.9
作者：
Huang, HD;Lee, TY;Tzeng, SW;Horng, JT
通讯作者：
Horng, JT
影响因子：
14.9
作者：
Obenauer, JC;Cantley, LC;Yaffe, MB
通讯作者：
Yaffe, MB