Identification of woolliness response genes in peach fruit after post-harvest treatments.
Identification of woolliness response genes in peach fruit after post-harvest treatments.
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DOI：
10.1093/jxb/ern069
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发表时间：
2008
影响因子：
6.9
通讯作者：
Cambiazo V
中科院分区：
文献类型：
--
作者：
González-Agüero M;Pavez L;Ibáñez F;Pacheco I;Campos-Vargas R;Meisel LA;Orellana A;Retamales J;Silva H;González M;Cambiazo V
毛糙是桃和油桃的一种生理障碍，当果实在长时间冷藏后成熟时变得明显。这种疾病具有商业重要性，因为将桃子运输到遥远的市场和销售前的储存需要低温。然而，关于桃绵状毛的分子基础的知识仍然是不完整的。为了解决这个问题，尼龙宏阵列包含847个非冗余表达序列标签（EST）从成熟的桃果实cDNA文库的开发和使用。桃（Prunus persica cv. O'Henry）在21 °C下成熟7d（多汁果实）与在4 °C下贮藏15d后再在21 °C下成熟7d（绵毛果实）进行比较。结果表明，在多汁果实和绵状果实中，共有106个基因存在差异表达。数据分析表明，这些基因中的大多数（>90%）的活性在羊毛状果实中受到抑制。在冷藏桃（CV。O'Henry），所选基因（眼镜蛇，内切多聚半乳糖醛酸酶，肉桂酰辅酶A还原酶，和rab11）的表达水平低于多汁的水果，并保持低绵毛桃成熟后，这是保守的绵毛果实从其他两个商业品种（cv. Flamekist和CV。优雅的女士）。此外，本研究的结果表明，在果实绵状的分子变化涉及细胞壁代谢和内膜运输相关基因的表达变化。总体而言，这里报道的结果提供了不同收获后处理下桃果实的转录组活性的初步表征。
Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21 °C (juicy fruit) were compared with those of fruit stored for 15 d at 4 °C and then ripened for 7 d at 21 °C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.
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影响因子：
3.9
作者：
BUESCHER, RW;FURMANSKI, RJ
通讯作者：
FURMANSKI, RJ
影响因子：
4.3
作者：
Campos-Vargas, Reinaldo;Becerra, Oscar;Defilippi, Bruno G.
通讯作者：
Defilippi, Bruno G.
影响因子：
7.4
作者：
Brinker, M;van Zyl, L;von Arnold, S
通讯作者：
von Arnold, S
影响因子：
7.4
作者：
Baluska, F;Hlavacka, A;Volkmann, D
通讯作者：
Volkmann, D
影响因子：
4.9
作者：
Alexandersson, E;Saalbach, G;Kjellbom, P
通讯作者：
Kjellbom, P