Microsequence analysis of peptides and proteins. IV. Structural studies on human leukocyte interferons.
Microsequence analysis of peptides and proteins. IV. Structural studies on human leukocyte interferons.
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肽和蛋白质的微序列分析。
DOI：
10.1016/0003-2697(82)90522-x
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发表时间：
1982
影响因子：
2.9
通讯作者：
S. Pestka
中科院分区：
文献类型：
--
作者：
J. Shively;U. Del Valle;R. Blacher;D. Hawke;W. P. Levy;M. Rubinstein;S. Stein;C. McGregor;J. Tarnowski;R. Bartlett;D. Lee;S. Pestka
用微量测序法测定了三种主要的人白细胞干扰素的胰蛋白酶肽序列。通过与由含有白细胞干扰素编码插入物的重组体的DNA序列预测的氨基酸序列进行比较来比对肽。此外，还报道了通过重组DNA方法在大肠杆菌中产生的两种人白细胞干扰素的延长的NH 2-末端氨基酸序列。本报告展示了微测序方法在低纳摩尔和亚纳摩尔量的生物学目的蛋白质和肽中的应用。
The sequence of the tryptic peptides of three major species of human leukocyte interferon was determined by microsequencing procedures. The peptides were aligned by comparison with the amino acid sequences predicted by the DNA sequences of recombinants containing leukocyte interferon-coding inserts. In addition, extended NH2-terminal amino acid sequences of two human leukocyte interferons produced in Escherichia coli by recombinant DNA methodology are also reported. This report demonstrates application of microsequencing methodology to low nanomole and subnanomole amounts of proteins and peptides of biological interest.
采用等度高效液相色谱法快速分离氨基酸苯硫基乙内酰脲。
影响因子：
2.9
作者：
Tarr,GE
通讯作者：
Tarr,GE