Two distinct populations of projection neurons in the rat lateral parafascicular thalamic nucleus and their cholinergic responsiveness.
Two distinct populations of projection neurons in the rat lateral parafascicular thalamic nucleus and their cholinergic responsiveness.
复制标题
DOI：
10.1016/j.neuroscience.2009.04.043
复制
发表时间：
2009-08-04
期刊：
影响因子：
3.3
通讯作者：
Cox, C. L.
中科院分区：
文献类型：
--
作者：
Beatty, J. A.;Sylwestrak, E. L.;Cox, C. L.
外侧束旁核（IPf）是丘脑板内核的成员，其是特征性地向新皮质和基底神经节提供广泛投射并且与唤醒、感觉和运动功能相关的核的集合。最近，lPf神经元已被证明具有不同于其他皮质投射丘脑中继神经元的特性。我们进行了全细胞记录从lPf神经元使用在体外大鼠切片制备，并发现了两个不同的神经元亚型，区分不同的形态和生理特征：弥漫性和浓密。弥散神经元，这是以前描述的，是主要的神经元亚型（66%）。这些神经元有很少的，不良的分支，延长树突，很少表现出爆发样的动作电位放电，丘脑皮质中继神经元的一个普遍存在的功能。有趣的是，我们发现了一个较小的人口的浓密的神经元（34%），具有相似的形态和生理特征的初级感觉丘脑核的丘脑皮质中继神经元。与其他丘脑皮层中继神经元相比，毒蕈碱胆碱能受体的激活通过M2受体的激活在大多数lPf神经元中产生膜超极化（60%）。在少数的lPf神经元（33%），毒蕈碱激动剂通过激活主要M3受体产生膜去极化。毒蕈碱受体介导的作用不依赖于IPf神经元亚型（即，弥漫性或丛状神经元）;然而，胆碱能作用与具有不同传出靶点的lPf神经元相关。来自额叶皮质荧光珠注射的逆行标记的lPf神经元主要由浓密型lPf神经元（78%）组成，但更重要的是，所有这些神经元都被毒蕈碱激动剂去极化。另一方面，由纹状体注射标记的lPf神经元主要被毒蕈碱激动剂超极化（63%）。我们的研究结果表明，两个不同的亚群的lPf投射神经元，有趣的是lPf神经元响应不同的毒蕈碱受体激活的基础上，他们的轴突目标。
The lateral parafascicular nucleus (lPf) is a member of the intralaminar thalamic nuclei, a collection of nuclei that characteristically provides widespread projections to the neocortex and basal ganglia and is associated with arousal, sensory, and motor functions. Recently, lPf neurons have been shown to possess different characteristics than other cortical-projecting thalamic relay neurons. We performed whole cell recordings from lPf neurons using an in vitro rat slice preparation and found two distinct neuronal subtypes that were differentiated by distinct morphological and physiological characteristics: diffuse and bushy. Diffuse neurons, which had been previously described, were the predominant neuronal subtype (66%). These neurons had few, poorly-branching, extended dendrites, and rarely displayed burst-like action potential discharge, a ubiquitous feature of thalamocortical relay neurons. Interestingly, we discovered a smaller population of bushy neurons (34%) that shared similar morphological and physiological characteristics with thalamocortical relay neurons of primary sensory thalamic nuclei. In contrast to other thalamocortical relay neurons, activation of muscarinic cholinergic receptors produced a membrane hyperpolarization via activation of M2 receptors in most lPf neurons (60%). In a minority of lPf neurons (33%), muscarinic agonists produced a membrane depolarization via activation of predominantly M3 receptors. The muscarinic receptor-mediated actions were independent of lPf neuronal subtype (i.e., diffuse or bushy neurons); however the cholinergic actions were correlated with lPf neurons with different efferent targets. Retrogradely-labeled lPf neurons from frontal cortical fluorescent bead injections primarily consisted of bushy type lPf neurons (78%), but more importantly, all of these neurons were depolarized by muscarinic agonists. On the other hand, lPf neurons labeled by striatal injections were predominantly hyperpolarized by muscarinic agonists (63%). Our results indicate two distinct subpopulations of lPf projection neurons, and interestingly lPf neurons respond differentially to muscarinic receptor activation based on their axonal target.
登录
查看更多内容
影响因子：
2.9
作者：
KINCAID, AE;PENNEY, JB;NEWMAN, SW
通讯作者：
NEWMAN, SW
影响因子：
2.5
作者：
DUBE, L;SMITH, AD;BOLAM, JP
通讯作者：
BOLAM, JP
影响因子：
2.5
作者：
FRANCESCONI, W;MULLER, CM;SINGER, W
通讯作者：
SINGER, W
影响因子：
5.3
作者：
Lacey, Carolyn J.;Bolam, J. Paul;Magill, Peter J.
通讯作者：
Magill, Peter J.
影响因子：
5.5
作者：
JAHNSEN, H;LLINAS, R
通讯作者：
LLINAS, R