T cell and APC dynamics in situ control the outcome of vaccination.
T cell and APC dynamics in situ control the outcome of vaccination.
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DOI：
10.4049/jimmunol.0901047
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发表时间：
2010-07-01
期刊：
影响因子：
--
通讯作者：
Lefrançois L
中科院分区：
文献类型：
--
作者：
Khanna KM;Blair DA;Vella AT;McSorley SJ;Datta SK;Lefrançois L
The factors controlling the progression of an immune response to generation of protective memory are poorly understood. We compared the in situ and ex vivo characteristics of CD8 T cells responding to different forms of the same immunogen. Immunization with live Listeria monocytogenes, irradiated L. monocytogenes (IRL), or heat-killed L. monocytogenes (HKL) induced rapid activation of CD8 T cells. However, only IRL and live L. monocytogenes inoculation induced sustained proliferation and supported memory development. Gene and protein expression analysis revealed that the three forms of immunization led to three distinct transcriptional and translational programs. Prior to cell division, CD8 T cell–dendritic cell clusters formed in the spleen after live L. monocytogenes and IRL but not after HKL immunization. Furthermore, HKL immunization induced rapid remodeling of splenic architecture, including loss of marginal zone macrophages, which resulted in impaired bacterial clearance. These results identify initial characteristics of a protective T cell response that have implications for the development of more effective vaccination strategies.
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影响因子：
56.9
作者：
Garside, P;Ingulli, E;Jenkins, MK
通讯作者：
Jenkins, MK
DOI：
10.1084/jem.175.6.1531
发表时间：
1992-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Harty JT;Bevan MJ
通讯作者：
Bevan MJ
DOI：
10.1073/pnas.0909541107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Backer, Ronald;Schwandt, Timo;den Haan, Joke M. M.
通讯作者：
den Haan, Joke M. M.
影响因子：
82.9
作者：
Brockstedt, DG;Bahjat, KS;Dubensky, TW
通讯作者：
Dubensky, TW
影响因子：
4.4
作者：
Edelson, BT;Unanue, ER
通讯作者：
Unanue, ER