New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments.
New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments.
复制标题
DOI：
10.3390/microorganisms9010030
复制
发表时间：
2020-12-24
期刊：
影响因子：
4.5
通讯作者：
Alain K
中科院分区：
文献类型：
--
作者：
Cozannet M;Borrel G;Roussel E;Moalic Y;Allioux M;Sanvoisin A;Toffin L;Alain K
古细菌Methanomassiliicoccales是主要与动物消化道相关的产甲烷菌。然而，环境成员的特征仍然很差，因为到目前为止还没有培养出与宿主无关的代表。在本研究中，对不同的非宿主相关环境样本进行元条形码筛选和定量PCR分析表明，Methanomassiliicoccales在大多数陆地和水生生态系统中非常稀缺。在环境浆液培养过程中，监测了产甲烷菌和产甲烷底物/产物的相对丰度。从淡水样品中获得了一种富含甲烷硅酸钙的沉积物浆液。由此构建了一个高质量的宏基因组组装基因组（MAG），并将其命名为候选种Methanomassiliicoccus armoricus MXMAG1。将MXMAG1的注释基因组与Methanomassiliicoccales属于2个已知分支（“自由生活”/非宿主相关环境分支和“宿主相关”/消化分支）的已发表基因组和MAGs进行比较，使我们能够探索候选datus ' M的假定生理特性。armoricus MXMAG1”。正如预期的那样，Ca. ‘ Methanomassiliicoccus armoricus MXMAG1 ’具有通过甲基化合物还原和二氢氧化产生甲烷的遗传潜力。该MAG编码了几种假定的生理和应激反应适应，包括海藻糖的生物合成（渗透和温度调节），agmatine的生产（pH调节），以及砷的解毒（通过亚砷酸盐的还原和排泄），这一机制仅存在于“自由生活”进化枝中。对环境样本和浆液的共生网络分析也表明，在陆地和水生生态系统中检测到的Methanomassiliicoccales与消化栖息地中常见的醋酸盐和二氢产菌密切相关，据报道，这些细菌与产甲烷菌形成共生关系。
Members of the archaeal order Methanomassiliicoccales are methanogens mainly associated with animal digestive tracts. However, environmental members remain poorly characterized as no representatives not associated with a host have been cultivated so far. In this study, metabarcoding screening combined with quantitative PCR analyses on a collection of diverse non-host-associated environmental samples revealed that Methanomassiliicoccales were very scarce in most terrestrial and aquatic ecosystems. Relative abundance of Methanomassiliicoccales and substrates/products of methanogenesis were monitored during incubation of environmental slurries. A sediment slurry enriched in Methanomassiliicoccales was obtained from a freshwater sample. It allowed the reconstruction of a high-quality metagenome-assembled genome (MAG) corresponding to a new candidate species, for which we propose the name of Candidatus ‘Methanomassiliicoccus armoricus MXMAG1’. Comparison of the annotated genome of MXMAG1 with the published genomes and MAGs from Methanomassiliicoccales belonging to the 2 known clades (‘free-living’/non-host-associated environmental clade and ‘host-associated’/digestive clade) allowed us to explore the putative physiological traits of Candidatus ‘M. armoricus MXMAG1’. As expected, Ca. ‘Methanomassiliicoccus armoricus MXMAG1’ had the genetic potential to produce methane by reduction of methyl compounds and dihydrogen oxidation. This MAG encodes for several putative physiological and stress response adaptations, including biosynthesis of trehalose (osmotic and temperature regulations), agmatine production (pH regulation), and arsenic detoxication, by reduction and excretion of arsenite, a mechanism that was only present in the ‘free-living’ clade. An analysis of co-occurrence networks carried out on environmental samples and slurries also showed that Methanomassiliicoccales detected in terrestrial and aquatic ecosystems were strongly associated with acetate and dihydrogen producing bacteria commonly found in digestive habitats and which have been reported to form syntrophic relationships with methanogens.
登录
查看更多内容
影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
珠江口盆地沉积物中微生物群落组成及功能
影响因子：
1.6
作者：
Chen Ye;Mi Tiezhu;Zhen Yu
通讯作者：
Zhen Yu
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
4.1
作者：
Azizi, Aqil;Kim, Wonduck;Lee, Jung Hyun
通讯作者：
Lee, Jung Hyun
DOI：
10.1155/2014/374146
发表时间：
2014
期刊：
Archaea (Vancouver, B.C.)
影响因子：
--
作者：
Borrel G;Gaci N;Peyret P;O'Toole PW;Gribaldo S;Brugère JF
通讯作者：
Brugère JF