Schizosaccharomyces pombe Ppr10 and Mpa1 together mediate mitochondrial translational initiation.
Schizosaccharomyces pombe Ppr10 and Mpa1 together mediate mitochondrial translational initiation.
复制标题
粟酒裂殖酵母 Ppr10 和 Mpa1 共同介导线粒体翻译起始
DOI：
10.1016/j.jbc.2021.100869
复制
发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Luo Y;Wang Y;Huang Y
五肽重复序列（PPR）蛋白是一个大家族的蛋白质，主要作用于细胞器基因表达的不同转录后步骤。我们以前已经发现，裂殖酵母PPR蛋白mpal 10与线粒体翻译激活因子Mpa 1相互作用，两者都是线粒体蛋白质合成所必需的。然而，目前还不清楚这两种蛋白质如何在粟酒裂殖酵母的线粒体蛋白质合成中发挥作用。在这项研究中，我们进一步研究了Ppr 10和Mpa 1在线粒体蛋白质合成中的作用。线粒体翻译起始需要两个起始因子，Mti 2和Mti 3，其在线粒体翻译起始复合物形成期间结合到线粒体核糖体的小亚基（mt-SSU）。使用蔗糖梯度沉降分析，我们发现，破坏的ppr 10，mpa 1，或PPR基序Ppr 10损害协会的Mti 2和Mti 3与mt-SSU，这表明Ppr 10和Mpa 1可能需要Mti 2和Mti 3与mt-SSU在组装线粒体翻译起始复合物。Ppr 10的缺失扰乱了线粒体编码的细胞色素B（cob 1）和细胞色素c氧化酶亚基1（cox 1）mRNA与组装的线粒体核糖体的结合。蛋白质组学分析表明，一部分的PPR 10和MPA 1 copurified与一个子集的线粒体蛋白质。Ppr 10的PPR基序对于其与Mpa 1的相互作用是必需的，并且这些PPR基序的破坏损害线粒体蛋白质合成。我们的研究结果表明，Ppr 10和Mpa 1功能一起介导线粒体翻译起始。
Pentatricopeptide repeat (PPR) proteins are a large family of proteins that act primarily at different posttranscriptional steps of organellar gene expression. We have previously found that the Schizosaccharomyces pombe PPR protein mpal10 interacts with mitochondrial translational activator Mpa1, and both are essential for mitochondrial protein synthesis. However, it is unclear how these two proteins function in mitochondrial protein synthesis in S. pombe. In this study, we further investigated the role of Ppr10 and Mpa1 in mitochondrial protein synthesis. Mitochondrial translational initiation requires two initiation factors, Mti2 and Mti3, which bind to the small subunit of the mitochondrial ribosome (mt-SSU) during the formation of the mitochondrial translational initiation complex. Using sucrose gradient sedimentation analysis, we found that disruption of ppr10, mpa1, or the PPR motifs in Ppr10 impairs the association of Mti2 and Mti3 with the mt-SSU, suggesting that both Ppr10 and Mpa1 may be required for the interaction of Mti2 and Mti3 with the mt-SSU during the assembly of mitochondrial translational initiation complex. Loss of Ppr10 perturbs the association of mitochondrially encoded cytochrome b (cob1) and cytochrome c oxidase subunit 1 (cox1) mRNAs with assembled mitochondrial ribosomes. Proteomic analysis revealed that a fraction of Ppr10 and Mpa1 copurified with a subset of mitoribosomal proteins. The PPR motifs of Ppr10 are necessary for its interaction with Mpa1 and that disruption of these PPR motifs impairs mitochondrial protein synthesis. Our results suggest that Ppr10 and Mpa1 function together to mediate mitochondrial translational initiation.
登录
查看更多内容
影响因子：
4.1
作者：
Lightowlers RN;Chrzanowska-Lightowlers ZM
通讯作者：
Chrzanowska-Lightowlers ZM
影响因子：
4.6
作者：
Kuzmenko A;Derbikova K;Salvatori R;Tankov S;Atkinson GC;Tenson T;Ott M;Kamenski P;Hauryliuk V
通讯作者：
Hauryliuk V
影响因子：
4.5
作者：
Metodiev MD;Spåhr H;Loguercio Polosa P;Meharg C;Becker C;Altmueller J;Habermann B;Larsson NG;Ruzzenente B
通讯作者：
Ruzzenente B
影响因子：
29
作者：
De Silva D;Fontanesi F;Barrientos A
通讯作者：
Barrientos A
影响因子：
5.4
作者：
Luo, Ying;Su, Ruyue;Huang, Ying
通讯作者：
Huang, Ying