De novo mRNA synthesis is required for both consolidation and reconsolidation of fear memories in the amygdala.
De novo mRNA synthesis is required for both consolidation and reconsolidation of fear memories in the amygdala.
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DOI：
10.1101/lm.1027208
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
LeDoux JE
中科院分区：
文献类型：
--
作者：
Duvarci S;Nader K;LeDoux JE
记忆巩固是将新学到的信息稳定到长期记忆中的过程。相当多的证据表明，恢复巩固的记忆会使它回到一个不稳定的状态，需要重新稳定。新的恐惧记忆的巩固已经被证明需要杏仁核（LA）外侧核中的RNA和蛋白质的重新合成。我们以前已经表明，从头蛋白质合成的LA是需要重新巩固听觉恐惧记忆。一个关键的问题是，在再巩固过程中，蛋白质的合成是否取决于杏仁核中已有的mRNA或新mRNA的合成。在本研究中，我们研究了mRNA合成抑制在听觉恐惧记忆巩固和再巩固过程中的作用。我们首先表明，LA内输注两种不同的mRNA抑制剂剂量依赖性损害长期记忆（LTM），但短期记忆（STM）完好无损。接下来，我们表明，LA内输注相同的抑制剂剂量依赖性地阻断再激活后的长期记忆（PR-LTM），而再激活后的短期记忆（PR-STM）是完整的。此外，在没有记忆再激活的情况下，同样的治疗没有效果。总之，这些结果表明，巩固和再巩固的听觉恐惧记忆需要从头mRNA合成，并同样敏感的从头mRNA合成的LA中断。
Memory consolidation is the process by which newly learned information is stabilized into long-term memory. Considerable evidence indicates that retrieval of a consolidated memory returns it to a labile state that requires it to be restabilized. Consolidation of new fear memories has been shown to require de novo RNA and protein synthesis in the lateral nucleus of the amygdala (LA). We have previously shown that de novo protein synthesis in the LA is required for reconsolidation of auditory fear memories. One key question is whether protein synthesis during reconsolidation depends on already existing mRNAs or on synthesis of new mRNAs in the amygdala. In the present study, we examined the effect of mRNA synthesis inhibition during consolidation and reconsolidation of auditory fear memories. We first show that intra-LA infusion of two different mRNA inhibitors dose-dependently impairs long-term memory (LTM), but leaves short-term memory (STM) intact. Next, we show that intra-LA infusion of the same inhibitors dose-dependently blocks post-reactivation long-term memory (PR-LTM), whereas post-reactivation short-term memory (PR-STM) is left intact. Furthermore, the same treatment in the absence of memory reactivation has no effect. Together, these results show that both consolidation and reconsolidation of auditory fear memories require de novo mRNA synthesis and are equally sensitive to disruption of de novo mRNA synthesis in the LA.
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影响因子：
1.9
作者：
Bailey, DJ;Kim, JJ;Helmstetter, FJ
通讯作者：
Helmstetter, FJ
DOI：
10.1037/h0027676
发表时间：
1969-01-01
期刊：
JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY
影响因子：
--
作者：
BLANCHAR.RJ;BLANCHAR.DC
通讯作者：
BLANCHAR.DC
影响因子：
25
作者：
Kida, S;Josselyn, SA;Silva, AJ
通讯作者：
Silva, AJ
影响因子：
64.8
作者：
Nader, K;Schafe, GE;Le Doux, JE
通讯作者：
Le Doux, JE
影响因子：
14.9
作者：
DREYER, C;HAUSEN, P
通讯作者：
HAUSEN, P