Epistatic Modifiers Influence the Expression of Continual Flowering in Strawberry
Epistatic Modifiers Influence the Expression of Continual Flowering in Strawberry
复制标题
上位修饰剂影响草莓持续开花的表达
DOI：
10.1101/2022.03.15.484381
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Cockerton H
中科院分区：
文献类型：
--
作者：
Cockerton H
社会影响声明直到20世纪70年代，大多数商业草莓品种只开一朵花。然而，不断开花、常结果实的草莓现在被常规种植，而且使用量正在增加。事实上，导入长花性状可以通过从同一植株连续生产一种作物来为种植者带来经济利益。基因引导的改良具有简化生育后代的能力。因此，这里报告的遗传标记可以帮助在育种过程的早期时间点识别永生个体。此外，这些标记可以帮助提高后代分离比的预测。概述以前在群落内的工作导致了控制永生性状的单一显性等位基因的识别。然而，频繁的观察表明，杂交并不像预期的由单个显性基因控制的性状那样以孟德尔式的方式分离。因此，假设有一个或多个未知的上位性等位基因与主效基因互作。对587个六月结实者和207个长结实者进行了全基因组关联研究，以评估与开花习性相关的遗传成分。利用表型已知的亲本草莓品系的分离比来验证所识别的等位基因，共鉴定出3个座位，包括已知的主效FAPFRU微基因座和2个上位性修饰基因。这些修饰符在含有FAPFU等位基因单一拷贝的个体中起到了永久生育特征的增强作用，并且似乎在功能上是多余的。基本上，杂合子个体需要两个修饰等位基因的存在才能表达永生基因。上位性等位基因的包含改善了永生分离比的预测；然而，除了单等位基因模型之外，很大一部分变异仍然无法解释。未来的工作应该确定这里没有确定的其他阻遏子和增强子修饰物。发现控制长寿性状的遗传成分可以使遗传知情草莓改良成为可能。
Society Impact StatementUntil the 1970s, the majority of commercial strawberry varieties produced a single bloom of flowers. However, continuously flowering, everbearing strawberries are now routinely cultivated and use is increasing. Indeed, introgression of the everbearing flowering trait can lead to economic benefits for growers through the production of a continual crop from the same plant. Genetically guided improvement has the power to streamline everbearing generation. As such, the genetic markers reported here can help to identify everbearing individuals at an early time point in the breeding process. Furthermore, these markers can help to improve the predictions of progeny segregation ratios.SummaryPrevious work within the community led to the identification of a single dominant allele that controls the everbearing trait. However, frequent observations have indicated that crosses do not segregate in a Mendelian fashion, as would be expected for a trait controlled by a single dominant gene. Therefore, it was hypothesised that one or more unidentified epistatic alleles interact with the major gene.A genome‐wide association study (GWAS) was conducted on 587 June bearers and 207 everbearers to assess the genetic components associated with flowering habit. The segregation ratios of parental strawberry lines with known phenotypes were used to validate the identified alleles.Three loci including the known majorFaPFRUlocus and two epistatic modifiers were identified. These modifiers function as enhancers of the everbearing trait in individuals containing a single copy of theFaPFRUeverbearing allele and appear to be functionally redundant. Principally, heterozygous individuals required the presence of two modifying alleles in order to allow expression of the everbearing trait.Inclusion of the epistatic alleles improved the prediction of everbearing segregation ratios; beyond that of a single allele model, however, a large proportion of the variation remained unexplained. Future work should identify the additional repressor and enhancer modifiers not identified here. Discovering the genetic components controlling the everbearing trait can enable genetic informed strawberry improvement.
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影响因子：
1.7
作者：
Sonali Mookerjee;Megan M. Mathey;C. Finn;Zhongna Zhang;J. Hancock
通讯作者：
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DOI：
10.21273/jashs.135.2.102
发表时间：
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影响因子：
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作者：
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影响因子：
6.9
作者：
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通讯作者：
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影响因子：
1
作者：
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通讯作者：
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