Solid-state NMR reveals differential carbohydrate utilization in diapausing Culex pipiens.
Solid-state NMR reveals differential carbohydrate utilization in diapausing Culex pipiens.
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DOI：
10.1038/srep37350
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发表时间：
2016-11-17
影响因子：
4.6
通讯作者：
Kim SJ
中科院分区：
文献类型：
--
作者：
Chang J;Singh J;Kim S;Hockaday WC;Sim C;Kim SJ
在北美，库蚊是西尼罗河病毒和其他人类致病性黄病毒的载体。对昼长缩短和温度降低的响应，雌性Cx；管道蜂通过主动取食碳水化合物为滞育做准备，增加糖原和脂质的生物合成，为越冬储存能量。采用13C固体核磁共振（NMR）技术研究了饲喂不同碳水化合物对滞育蚊体内糖原和脂质生物合成的影响。在成虫羽化后，将滞育雌虫和未滞育雌虫分别饲喂3种不同的碳水化合物，分别饲喂7天：1)10%蔗糖，2)10% D-[13C6]葡萄糖，3)1% D-[13C6]葡萄糖和10%蔗糖。核磁共振测量表明，蔗糖和葡萄糖在滞育蚊子体内的代谢方式不同。以蔗糖为食的蚊子主要积累糖原，其分支结构增加，但脂质较少。相反，仅以葡萄糖为食的蚊子糖原和脂质均有积累，脂肪链长度增加。当蚊子与蔗糖共同喂食时，葡萄糖被专门代谢为三酰甘油的生物合成。我们的发现为昆虫滞育过程中控制糖原和脂质生物合成的碳水化合物代谢提供了新的见解，这是昆虫在动物环境中生存的基础。
Culex pipiens is the mosquito that vectors West Nile Virus and other human-pathogenic flavivruses in North America. In response to shortened day length and lower temperatures, female Cx. pipiense prepares for the diapause by actively feeding on carbohydrates to increase the biosynthesis of glycogen and lipid to store energy for overwintering. The effect of feeding different carbohydrates on glycogen and lipid biosynthesis in diapausing mosquitoes was investigated in vivo using 13C solid-state NMR. Diapause-destined adult females and nondiapausing counterparts after adult eclosion were fed with three different carbohydrate sources for 7 days: 1) 10% sucrose, 2) 10% D-[13C6]glucose, and 3) 1% D-[13C6]glucose co-provisioned with 10% sucrose. NMR measurements show that sucrose and glucose are metabolized differently in diapausing mosquitoes. Mosquitoes fed on sucrose primarily accumulate glycogen with increased branching structures, but less of lipids. In contrast, mosquitoes fed exclusively on glucose show accumulation of both glycogen and lipid with increased aliphatic chain length. Glucose is exclusively metabolized for the biosynthesis of triacylglyceride when mosquitoes were co-fed with sucrose. Our findings provide novel insights into the insect carbohydrate metabolism that governs glycogen and lipid biosynthesis during diapause, which is fundamental for the insect survival during inimical environments.
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影响因子：
64.5
作者：
Miyamoto T;Slone J;Song X;Amrein H
通讯作者：
Amrein H
影响因子：
2.1
作者：
REISEN, WK;MEYER, RP;MILBY, MM
通讯作者：
MILBY, MM
影响因子：
4.6
作者：
Sim, Cheolho;Denlinger, David L.
通讯作者：
Denlinger, David L.
影响因子：
4.4
作者：
BENNETT, AE;RIENSTRA, CM;GRIFFIN, RG
通讯作者：
GRIFFIN, RG
DOI：
10.1016/0020-1790(90)90074-5
发表时间：
1990-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
MARINOTTI, O;JAMES, AA
通讯作者：
JAMES, AA