A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba
A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba
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产白果内酯的内生真菌，来自药用植物银杏的多盘拟盘菌
DOI：
10.1007/s00284-016-1060-6
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发表时间：
2016-05
影响因子：
2.6
通讯作者：
Kang JC
中科院分区：
文献类型：
--
作者：
Qian Yi-Xin;Kang Ji-Chuan;Zhao Jun-Jie;He Jun;Kang Ji-Chuan;Zhao Jun-Jie;Luo Yi-Kai;Geng Kun;Kang JC
从药用植物银杏（Ginkgo biloba）的茎、根、叶和树皮中分离到57株产银杏内酯（BB）的内生真菌。biloba.发酵工艺使用除G. biloba可能成为生产BB的新途径，BB是一种具有神经保护作用的萜烯三内酯。本研究从银杏叶片中分离到一株产BB的内生真菌GZUYX 13。中国贵州省贵阳市贵州大学校园内生长的银杏。该菌株在马铃薯葡萄糖液体培养基中生长时产生BB。通过高效液相色谱（HPLC）定量该内生真菌产生的BB的量为106 μg/L，显著低于宿主组织产生的BB的量。经薄层色谱和高效液相色谱分析，证实该真菌BB与真实BB相同。经形态学观察和ITS rDNA序列分析，将菌株GZUYX 13鉴定为悬铃木拟盘多毛孢（Pestalotiopsis uvicola）。据我们所知，这是第一次报道关于内生产BB的拟盘多毛孢属的分离和鉴定。进一步证明了内生真菌具有产生生物活性物质的潜力。
For screening bilobalide (BB)-producing endophytic fungi from medicinal plant Ginkgo biloba, a total of 57 fungal isolates were isolated from the internal stem, root, leaf, and bark of the plant G. biloba. Fermentation processes using BB-producing fungi other than G. biloba may become a novel way to produce BB, which is a terpene trilactones exhibiting neuroprotective effects. In this study, a BB-producing endophytic fungal strain GZUYX13 was isolated from the leaves of G. biloba grown in the campus of Guizhou University, Guiyang city, Guizhou province, China. The strain produced BB when grown in potato dextrose liquid medium. The amount of BB produced by this endophytic fungus was quantified to be 106 μg/L via high-performance liquid chromatography (HPLC), substantially lower than that produced by the host tissue. The fungal BB which was analyzed by thin layer chromatography (TLC) and HPLC was proven to be identical to authentic BB. The strain GZUYX13 was identified as Pestalotiopsis uvicola via morphology and ITS rDNA phylogeny. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic BB-producing Pestalotiopsis spp. from the host plant, which further proved that endophytic fungi have the potential to produce bioactive compounds.
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DOI：
--
发表时间：
2002
期刊：
Journal of Xiamen University
影响因子：
--
作者：
Huang Yao-jian
通讯作者：
Huang Yao-jian
影响因子：
4.1
作者：
Higgins, K. Lindsay;Arnold, A. Elizabeth;Lutzoni, Francois
通讯作者：
Lutzoni, Francois
影响因子：
15
作者：
CRIMMINS, MT;JUNG, DK;GRAY, JL
通讯作者：
GRAY, JL
影响因子：
5
作者：
Shelley H. Huang;R. Duke;M. Chebib;K. Sasaki;K. Wada;G. Johnston
通讯作者：
Shelley H. Huang;R. Duke;M. Chebib;K. Sasaki;K. Wada;G. Johnston
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M