Wolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics.
Wolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics.
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DOI：
10.1186/1471-2148-13-31
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发表时间：
2013-02-05
影响因子：
3.4
通讯作者：
Caccone A
中科院分区：
文献类型：
--
作者：
Symula RE;Alam U;Brelsfoard C;Wu Y;Echodu R;Okedi LM;Aksoy S;Caccone A
Wolbachia pipientis是一组不同的α-变形菌，可以改变节肢动物宿主的繁殖，并赋予Wolbachia感染的雌性生殖优势（细胞质不相容性（CI））。这种优势可以改变宿主群体遗传学，因为感染沃尔巴克氏体的雌性比未感染的雌性产生更多具有自己线粒体DNA（mtDNA）单倍型的后代。因此，这些宿主单倍型变得常见或固定（选择性扫描）。虽然模拟表明，CI介导的扫描发生，必须有一个短暂的阶段，重复初始感染的多个不同的沃尔巴克氏体菌株的主机，这还没有观察到经验。Wolbachia已经在采采蝇Glossina fuscipes fuscipes中发现，但它不限于单一宿主单倍型，这表明CI没有影响其种群结构。然而，宿主群体的遗传分化可能已经产生，如果多个沃尔巴克氏体菌株在某些人群中相互作用。在这里，我们调查了沃尔巴克氏体遗传变异的G。F.已知的宿主遗传组成的fuscipes种群在乌干达。我们使用多位点序列分型（MLST）和地理区域和宿主mtDNA单倍型之间的关联使用沃尔巴克氏体DNA序列从一个可变的位点，groEL（热休克蛋白60）的存在下，多个沃尔巴克氏体菌株进行了测试。MLST结果表明，部分G. F.梭形虫携带来自两个谱系的沃尔巴克氏体菌株。GroEL揭示了个体内和个体间的高水平序列多样性（单倍型多样性= 0.945）。我们发现沃尔巴克氏体与26个宿主mtDNA单倍型相关，这是一个前所未有的结果。我们观察到一个沃尔巴克氏体谱系与南方宿主mtDNA单倍型的地理关联，但不显著（p = 0.16）。虽然大多数沃尔巴克氏体感染的宿主单倍型是在宿主mtDNA组之间的接触区域发现的，但这种关联不显著（p = 0.17）。沃尔巴克氏体序列的高度多样性以及沃尔巴克氏体与多种宿主单倍型的关联表明，不同的沃尔巴克氏体菌株感染G。F.多次独立融合。我们认为，这些意见反映了一个短暂的阶段，沃尔巴克氏体的演变是由长期妊娠和低繁殖输出的采采蝇的影响。尽管G. F. fuscipes是重叠感染沃尔巴克氏体，我们的数据不支持双向CI影响宿主遗传多样性在乌干达。
Wolbachia pipientis, a diverse group of α-proteobacteria, can alter arthropod host reproduction and confer a reproductive advantage to Wolbachia-infected females (cytoplasmic incompatibility (CI)). This advantage can alter host population genetics because Wolbachia-infected females produce more offspring with their own mitochondrial DNA (mtDNA) haplotypes than uninfected females. Thus, these host haplotypes become common or fixed (selective sweep). Although simulations suggest that for a CI-mediated sweep to occur, there must be a transient phase with repeated initial infections of multiple individual hosts by different Wolbachia strains, this has not been observed empirically. Wolbachia has been found in the tsetse fly, Glossina fuscipes fuscipes, but it is not limited to a single host haplotype, suggesting that CI did not impact its population structure. However, host population genetic differentiation could have been generated if multiple Wolbachia strains interacted in some populations. Here, we investigated Wolbachia genetic variation in G. f. fuscipes populations of known host genetic composition in Uganda. We tested for the presence of multiple Wolbachia strains using Multi-Locus Sequence Typing (MLST) and for an association between geographic region and host mtDNA haplotype using Wolbachia DNA sequence from a variable locus, groEL (heat shock protein 60). MLST demonstrated that some G. f. fuscipes carry Wolbachia strains from two lineages. GroEL revealed high levels of sequence diversity within and between individuals (Haplotype diversity = 0.945). We found Wolbachia associated with 26 host mtDNA haplotypes, an unprecedented result. We observed a geographical association of one Wolbachia lineage with southern host mtDNA haplotypes, but it was non-significant (p = 0.16). Though most Wolbachia-infected host haplotypes were those found in the contact region between host mtDNA groups, this association was non-significant (p = 0.17). High Wolbachia sequence diversity and the association of Wolbachia with multiple host haplotypes suggest that different Wolbachia strains infected G. f. fuscipes multiple times independently. We suggest that these observations reflect a transient phase in Wolbachia evolution that is influenced by the long gestation and low reproductive output of tsetse. Although G. f. fuscipes is superinfected with Wolbachia, our data does not support that bidirectional CI has influenced host genetic diversity in Uganda.
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影响因子：
3.8
作者：
Dyer NA;Ravel S;Choi KS;Darby AC;Causse S;Kapitano B;Hall MJ;Steen K;Lutumba P;Madinga J;Torr SJ;Okedi LM;Lehane MJ;Donnelly MJ
通讯作者：
Donnelly MJ
影响因子：
3.8
作者：
Duron, O.;Fort, P.;Weill, M.
通讯作者：
Weill, M.
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
2.6
作者：
Dutton, TJ;Sinkins, SP
通讯作者：
Sinkins, SP
影响因子：
2.1
作者：
Flor, M.;Hammerstein, P.;Telschow, A.
通讯作者：
Telschow, A.