A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.
A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.
复制标题
一个基于微流体悬挂的胰岛渗漏系统，用于研究多个单个胰岛的葡萄糖刺激的胰岛素分泌。
DOI：
10.3389/fbioe.2021.674431
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发表时间：
2021
影响因子：
5.7
通讯作者：
Misun PM
中科院分区：
文献类型：
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作者：
Wu Jin P;Rousset N;Hierlemann A;Misun PM
胰岛灌注系统可用于在葡萄糖刺激胰岛素分泌 (GSIS) 测定中监测胰岛的高度动态胰岛素释放。在这里，我们提出了新一代基于微流体悬滴的胰岛灌注平台，该平台是为了在高时间分辨率下研究单个胰岛微组织胰岛素分泌动力学的变化而开发的。该平台经过彻底重新设计，以提高实验吞吐量并降低操作复杂性。通过实施互连的悬滴网络，实验吞吐量增加了四倍，该网络允许以 30 秒的采样间隔并行地对四个单独的胰岛微组织进行 GSIS 测定。我们引入了一种自动调节落差高度机制，可实现连续流动并保持芯片中恒定的液体体积，从而实现简单而稳健的操作。在葡萄糖刺激后，系统中所有胰岛同时观察到可重复的双相胰岛素释放。由于精确的液体处理、稳定的液滴体积、通道中的相等流速以及所有四个液滴中的精确控制的采样体积，测量的胰岛素浓度显示出较低的样品间差异。该装置将成为胰岛和糖尿病研究中的一个有价值的工具，用于研究单个胰岛的动态胰岛素分泌。
Islet perifusion systems can be used to monitor the highly dynamic insulin release of pancreatic islets in glucose-stimulated insulin secretion (GSIS) assays. Here, we present a new generation of the microfluidic hanging-drop-based islet perifusion platform that was developed to study the alterations in insulin secretion dynamics from single pancreatic islet microtissues at high temporal resolution. The platform was completely redesigned to increase experimental throughput and to reduce operational complexity. The experimental throughput was increased fourfold by implementing a network of interconnected hanging drops, which allows for performing GSIS assays with four individual islet microtissues in parallel with a sampling interval of 30 s. We introduced a self-regulating drop-height mechanism that enables continuous flow and maintains a constant liquid volume in the chip, which enables simple and robust operation. Upon glucose stimulation, reproducible biphasic insulin release was simultaneously observed from all islets in the system. The measured insulin concentrations showed low sample-to-sample variation as a consequence of precise liquid handling with stable drop volumes, equal flow rates in the channels, and accurately controlled sampling volumes in all four drops. The presented device will be a valuable tool in islet and diabetes research for studying dynamic insulin secretion from individual pancreatic islets.
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影响因子：
6.1
作者：
de Groot TE;Veserat KS;Berthier E;Beebe DJ;Theberge AB
通讯作者：
Theberge AB
影响因子：
--
作者：
Buchwald P
通讯作者：
Buchwald P
影响因子：
4.1
作者：
Misun PM;Yesildag B;Forschler F;Neelakandhan A;Rousset N;Biernath A;Hierlemann A;Frey O
通讯作者：
Frey O
影响因子：
7.4
作者：
Dishinger, John F.;Reid, Kendra R.;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.
DOI：
10.1039/c6an01055e
发表时间：
2016-10-21
期刊：
The Analyst
影响因子：
--
作者：
Brooks JC;Ford KI;Holder DH;Holtan MD;Easley CJ
通讯作者：
Easley CJ