Macro-to-micro interfacing to microfluidic channels using 3D-printed templates: application to time-resolved secretion sampling of endocrine tissue.
Macro-to-micro interfacing to microfluidic channels using 3D-printed templates: application to time-resolved secretion sampling of endocrine tissue.
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DOI：
10.1039/c6an01055e
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发表时间：
2016-10-21
期刊：
影响因子：
--
通讯作者：
Easley CJ
中科院分区：
文献类型：
--
作者：
Brooks JC;Ford KI;Holder DH;Holtan MD;Easley CJ
采用3D打印模板进行宏观到微观接口，设计了被动操作的聚二甲基硅氧烷（PDMS）微流体装置，用于从原代小鼠胰岛和附睾白色脂肪组织外植体进行时间分辨分泌物采样。类似装置中的接口通常通过手动穿孔或钻孔的流体贮存器来实现。我们之前介绍了使用手工制造的聚合物插入物将细胞培养物和采样储器模板化到PDMS装置中的概念，从而允许内分泌组织的快速刺激和采样。然而，流体贮存器的制造是耗时的、繁琐的，并且在装置固化期间易于出错。在这里，我们已经实现了计算机辅助设计和3D打印，以规避这些制造障碍。除了快速原型和设计迭代的优势外，将这些3D打印界面模板与通道图案相匹配的能力也非常有益。通过将模板制造过程数字化，可以生产更坚固的部件，同时降低制造可变性。在本文中，3D打印的模板用于在被动操作的八通道装置中将毫米级储库雕刻到上述通道的本体PDMS中，所述八通道装置被设计用于鼠组织的时间分辨分泌物采样。通过对<10个胰岛的葡萄糖刺激胰岛素分泌和2 mm脂肪组织外植体的甘油分泌进行时间分析，证明了设备的功能，这表明3D打印的界面模板可适用于各种细胞和组织类型。更一般地说，这项工作验证了桌面3D打印机作为微流体实验室中的通用接口工具。3D打印模板用于将设计特定的流体储存器雕刻到微流体装置的通道上方大块基底中，这些储存器用于小鼠胰岛和脂肪组织外植体的定制培养和时间分辨分泌物采样。
Employing 3D-printed templates for macro-to-micro interfacing, a passively operated polydimethysiloxane (PDMS) microfluidic device was designed for time-resolved secretion sampling from primary murine islets and epidiymal white adipose tissue explants. Interfacing in similar devices is typically accomplished through manually punched or drilled fluidic reservoirs. We previously introduced the concept of using hand fabricated polymer inserts to template cell culture and sampling reservoirs into PDMS devices, allowing rapid stimulation and sampling of endocrine tissue. However, fabrication of the fluidic reservoirs was time consuming, tedious, and was prone to errors during device curing. Here, we have implemented computer-aided design and 3D printing to circumvent these fabrication obstacles. In addition to rapid prototyping and design iteration advantages, the ability to match these 3D-printed interface templates with channel patterns is highly beneficial. By digitizing the template fabrication process, more robust components can be produced with reduced fabrication variability. Herein, 3D-printed templates were used for sculpting millimetre-scale reservoirs into the above-channel, bulk PDMS in passively-operated, eight-channel devices designed for time-resolved secretion sampling of murine tissue. Devices were proven functional by temporally assaying glucose-stimulated insulin secretion from <10 pancreatic islets and glycerol secretion from 2-mm adipose tissue explants, suggesting that 3D-printed interface templates could be applicable to a variety of cells and tissue types. More generally, this work validates desktop 3D printers as versatile interfacing tools in microfluidic laboratories. 3D-printed templates were used for sculpting design-specific fluidic reservoirs into the above-channel bulk substrate of microfluidic devices, and these reservoirs were used for customized culture and time-resolved secretion sampling of murine islets and adipose tissue explants.
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DOI：
10.1039/c4an01725k
发表时间：
2015-02-21
期刊：
The Analyst
影响因子：
--
作者：
Godwin LA;Brooks JC;Hoepfner LD;Wanders D;Judd RL;Easley CJ
通讯作者：
Easley CJ
影响因子：
15.9
作者：
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ORCI, L
影响因子：
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Kennedy, Robert T.
影响因子：
7.4
作者：
Gross, Bethany C.;Erkal, Jayda L.;Spence, Dana M.
通讯作者：
Spence, Dana M.
影响因子：
46.9
作者：
Bhatia, Sangeeta N.;Ingber, Donald E.
通讯作者：
Ingber, Donald E.