Localization of γ‐tubulin and cyclin B during early cleavage in physiologically polyspermic newt eggs
Localization of γ‐tubulin and cyclin B during early cleavage in physiologically polyspermic newt eggs
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生理多精蝾螈卵早期卵裂过程中 γ-微管蛋白和细胞周期蛋白 B 的定位
DOI：
10.1046/j.1440-169x.2002.00661.x
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发表时间：
2002
期刊：
影响因子：
4.6
通讯作者：
M. Yamashita
中科院分区：
文献类型：
--
作者：
Y. Iwao;T. Murakawa;J. Yamaguchi;M. Yamashita
为了了解蝾螈生理性多精卵中多精受阻的非常缓慢的机制，我们使用共聚焦激光显微镜确定了受精卵中γ-微管蛋白和细胞周期蛋白B1的分布。动物半球中的 γ-微管蛋白多于植物半球。主精子核和受精卵核的中心体有大量积累的γ微管蛋白，但副精子核中心体的γ微管蛋白很少。这些结果与最大的精子紫苑与主精子核相关的观察结果一致。在间期结束时，动物半球中出现的细胞周期蛋白 B1 多于植物半球中的细胞周期蛋白 B1。受精卵核内积累了大量的细胞周期蛋白B1，但与副精子核相关的细胞周期蛋白B1很少。细胞周期蛋白 B1 在中期受精卵核周围比在副精子核周围更早消失。这些发现与蝾螈卵中受精卵核比副精子核更早进入和退出中期相吻合，支持我们的成熟促进因子（MPF）模型，该模型解释了生理性多精卵核变性的机制。 Cyclin B1在同步分裂间期开始在细胞核内积累，其表达量最大的是前中期的中心体和细胞核。
To understand the mechanism of the very slow block to polyspermy in physiologically polyspermic eggs of the newt Cynops pyrrhogaster, we used confocal laser microscopy to determine the distribution of γ‐tubulin and cyclin B1 in fertilized eggs. More γ‐tubulin was localized in the animal hemisphere than in the vegetal. The centrosomes of the principal sperm nucleus and the zygote nucleus had much accumulated γ‐tubulin, but little γ‐tubulin was associated with the centrosomes of the accessory sperm nuclei. These results are consistent with observations that the largest sperm aster is associated with the principal sperm nucleus. More cyclin B1 appeared in the animal hemisphere than in the vegetal at the end of interphase. The zygote nucleus had much accumulated cyclin B1, but little cyclin B1 was associated with the accessory sperm nuclei. Cyclin B1 disappeared earlier around the zygote nucleus at metaphase than around the accessory sperm nuclei. These findings correspond well with the earlier entry and exit into metaphase in the zygote nucleus than in the accessory sperm nuclei in newt eggs, supporting our maturation‐promoting factor (MPF) model that accounts for the mechanism of nuclear degeneration in physiologically polyspermic eggs. Cyclin B1 began to accumulate in the nucleus during interphase in synchronous cleavage, and its greatest expression was in the centrosomes and the nucleus at prometaphase.
影响因子：
56.9
作者：
SAGATA, N;DAAR, I;VANDEWOUDE, GF
通讯作者：
VANDEWOUDE, GF
影响因子：
11.4
作者：
PINES, J;HUNTER, T
通讯作者：
HUNTER, T