Misregulation of ER-Golgi Vesicle Transport Induces ER Stress and Affects Seed Vigor and Stress Response.
Misregulation of ER-Golgi Vesicle Transport Induces ER Stress and Affects Seed Vigor and Stress Response.
复制标题
内质网-高尔基体囊泡运输的失调会引起内质网应激并影响种子活力和应激反应
DOI：
10.3389/fpls.2018.00658
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发表时间：
2018
影响因子：
5.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Zhao X;Guo X;Tang X;Zhang H;Wang M;Kong Y;Zhang X;Zhao Z;Lv M;Li L
高等植物的种子积累了大量的贮藏蛋白质，作为植物早期发育的氮源。种子贮藏蛋白（SSP）是在粗面内质网（rER）上合成的大型前体蛋白，通过囊泡运输进入蛋白贮藏泡（PSV），在那里加工成成熟蛋白。我们以前确定了一个拟南芥ER定位的拴系复合物，MAG 2复合物，这可能涉及高尔基体ER逆行运输。MAG 2复合物由4个亚基组成：MAG 2、MIP 1、MIP 2和MIP 3。这些亚基等位基因缺陷的突变体在内质网腔内积累SSP前体。在这里，我们报告了mag 2 -1 mip 3 -1和mip 2 -1 mip 3 -1双突变体比mag 2 -1、mip 2 -1和mip 3 -1单突变体具有更严重的囊泡转运缺陷，因为它们比相应的单突变体积累了更多的SSP前体，并且ER应激比单突变体更严重。mip 3 -1和mip 2 -1 mip 3 -1双突变体比单突变体表现出生长和发育缺陷。发现单突变体和双突变体种子都比野生型种子具有较低的蛋白质含量和降低的发芽势。所有突变体对盐和脱落酸（阿坝）胁迫敏感，阿坝信号途径发生改变。本研究阐明了内质网-高尔基体囊泡运输通过控制种子蛋白质质量和含量影响种子活力，以及通过影响阿坝信号通路影响植物对环境胁迫的响应。
Seeds of higher plants accumulate numerous storage proteins to use as nitrogen resources for early plant development. Seed storage proteins (SSPs) are synthesized as large precursors on the rough endoplasmic reticulum (rER), and are delivered to protein storage vacuoles (PSVs) via vesicle transport, where they are processed to mature forms. We previously identified an Arabidopsis ER-localized tethering complex, MAG2 complex, which might be involved in Golgi to ER retrograde transport. The MAG2 complex is composed of 4 subunits, MAG2, MIP1, MIP2, and MIP3. Mutants with defective alleles for these subunits accumulated SSP precursors inside the ER lumen. Here, we report that the mag2-1 mip3-1 and mip2-1 mip3-1 double mutant have more serious vesicle transport defects than the mag2-1, mip2-1, and mip3-1 single mutants, since they accumulate more SSP precursors than the corresponding single mutants, and ER stress is more severe than the single mutants. The mag2-1 mip3-1 and mip2-1 mip3-1 double mutants show growth and developmental defects rather than the single mutants. Both single and double mutant seeds are found to have lower protein content and decreased germinating vigor than wild type seeds. All the mutants are sensitive to abscisic acid (ABA) and salt stress, and exhibit alteration in ABA signaling pathway. Our study clarified that ER-Golgi vesicle transport affects seed vigor through controlling seed protein quality and content, as well as plant response to environmental stress via influencing ABA signaling pathway.
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影响因子：
4.2
作者：
Groot SP;Surki AA;de Vos RC;Kodde J
通讯作者：
Kodde J
影响因子：
11.4
作者：
Ballesteros, M;Fredriksson, Å;Nyström, T
通讯作者：
Nyström, T
影响因子：
64.8
作者：
HARMAN, GE;MATTICK, LR
通讯作者：
MATTICK, LR
影响因子：
11.4
作者：
BRAAKMAN, I;HELENIUS, J;HELENIUS, A
通讯作者：
HELENIUS, A
影响因子：
4.1
作者：
Das, N;Levine, RL;Sohal, RS
通讯作者：
Sohal, RS