Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions.
Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions.
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DOI：
10.1093/aob/mcs198
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发表时间：
2012-11
期刊：
影响因子：
4.2
通讯作者：
Kodde J
中科院分区：
文献类型：
--
作者：
Groot SP;Surki AA;de Vos RC;Kodde J
尽管干燥和相对潮湿的种子之间的生理差异，种子老化测试最经常使用的温度和种子水分水平高于在商业实践和基因库中使用的干燥储存期间。本研究旨在测试在干燥条件下种子老化是否可以通过在高压氧下储存来加速。将干大麦（Hordeum vulgare）、甘蓝（Brassica oleracea）、莴苣（Lactuca sativa）和大豆（Glycine max）种子在18 MPa氧分压下的钢罐中储存2至7周。在高压氮气或环境空气压力下储存作为对照。将该方法与在85%相对湿度下平衡后在45 °C下储存和在实验室工作台长期储存进行比较。发芽行为，幼苗形态和生育酚水平进行了评估。在高压氧下储存确实加速了干种子的老化。贮藏种子的形态老化症状与长期干燥贮藏条件下老化后观察到的症状相似。与莴苣和大豆相比，大麦对这种贮藏处理更耐受。不太成熟的收获的卷心菜种子更敏感，是引发的情况下，与非引发的生菜种子。在高压氧储存下，干燥种子的生育酚水平随着种子萌发的下降以线性方式下降，但在85%RH下平衡后在45 °C下储存期间劣化的种子中保持不变。种子在高压氧下贮藏提供了一种新的和相对快速的方法来研究种子在不同水分水平和温度下老化的生理和生物化学，包括那些代表基因库和商业实践中使用的干燥贮藏条件。
Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under dry conditions can be accelerated by storing under high-pressure oxygen. Dry barley (Hordeum vulgare), cabbage (Brassica oleracea), lettuce (Lactuca sativa) and soybean (Glycine max) seeds were stored between 2 and 7 weeks in steel tanks under 18 MPa partial pressure of oxygen. Storage under high-pressure nitrogen gas or under ambient air pressure served as controls. The method was compared with storage at 45 °C after equilibration at 85 % relative humidity and long-term storage at the laboratory bench. Germination behaviour, seedling morphology and tocopherol levels were assessed. The ageing of the dry seeds was indeed accelerated by storing under high-pressure oxygen. The morphological ageing symptoms of the stored seeds resembled those observed after ageing under long-term dry storage conditions. Barley appeared more tolerant of this storage treatment compared with lettuce and soybean. Less-mature harvested cabbage seeds were more sensitive, as was the case for primed compared with non-primed lettuce seeds. Under high-pressure oxygen storage the tocopherol levels of dry seeds decreased, in a linear way with the decline in seed germination, but remained unchanged in seeds deteriorated during storage at 45 °C after equilibration at 85 % RH. Seed storage under high-pressure oxygen offers a novel and relatively fast method to study the physiology and biochemistry of seed ageing at different seed moisture levels and temperatures, including those that are representative of the dry storage conditions as used in gene banks and commercial practice.
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影响因子：
7.2
作者：
Joosen, Ronny V. L.;Kodde, Jan;Hilhorst, Henk W. M.
通讯作者：
Hilhorst, Henk W. M.
影响因子：
7.4
作者：
Choi, Young Hae;van Spronsen, Jaap;Verpoorte, Robert
通讯作者：
Verpoorte, Robert
影响因子：
9.4
作者：
Bino, RJ;de Vos, CHR;Levin, I
通讯作者：
Levin, I
影响因子：
4.2
作者：
ELLIS, RH;ROBERTS, EH
通讯作者：
ROBERTS, EH
影响因子：
1.9
作者：
Demir, Ibrahim;Mavi, Kazim
通讯作者：
Mavi, Kazim