RecA-independent recombination: Dependence on the Escherichia coli RarA protein.
RecA-independent recombination: Dependence on the Escherichia coli RarA protein.
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DOI：
10.1111/mmi.14655
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发表时间：
2021-06
影响因子：
3.6
通讯作者：
Cox MM
中科院分区：
文献类型：
--
作者：
Jain K;Wood EA;Romero ZJ;Cox MM
大多数，但不是全部，细菌中的同源基因重组是由RecA重组酶介导的。不依赖reca的重组的机制起源仍然是个谜。在这里，我们证明了RarA蛋白对reca非依赖性重组有重要的酶促作用。特别是，RarA在分子间重组和涉及相对较短（< 200bp）同源序列的重组事件中做出了重大贡献，而reca介导的重组是低效的。这种效应在质粒重组实验和体内克隆过程中可见。即使在没有RecA和RarA的情况下，残留的重组水平仍然存在。reca非依赖性重组的其他途径，可能由解旋酶介导，被外切酶ExoI和RecJ抑制。翻译DNA聚合酶也可能起作用。我们的结果为之前关于RecA和RarA之间功能重叠的报道提供了额外的内容。
Most, but not all, homologous genetic recombination in bacteria is mediated by the RecA recombinase. The mechanistic origin of RecA-independent recombination has remained enigmatic. Here, we demonstrate that the RarA protein makes a major enzymatic contribution to RecA-independent recombination. In particular, RarA makes substantial contributions to intermolecular recombination and to recombination events involving relatively short (<200 bp) homologous sequences, where RecA-mediated recombination is inefficient. The effects are seen here in plasmid-based recombination assays and in vivo cloning processes. Vestigial levels of recombination remain even when both RecA and RarA are absent. Additional pathways for RecA-independent recombination, possibly mediated by helicases, are suppressed by exonucleases ExoI and RecJ. Translesion DNA polymerases may also contribute. Our results provide additional substance to a previous report of a functional overlap between RecA and RarA.
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影响因子：
64.8
作者：
Cox, MM;Goodman, MF;Marians, KJ
通讯作者：
Marians, KJ
DOI：
10.1073/pnas.111008998
发表时间：
2001-07-17
影响因子：
11.1
作者：
Barre, FX;Soballe, B;Sherratt, D
通讯作者：
Sherratt, D
影响因子：
4.8
作者：
Gabbai, Carolina B.;Yeeles, Joseph T. P.;Marians, Kenneth J.
通讯作者：
Marians, Kenneth J.
影响因子：
4.5
作者：
Costes A;Lecointe F;McGovern S;Quevillon-Cheruel S;Polard P
通讯作者：
Polard P
影响因子：
4.6
作者：
García-Nafría J;Watson JF;Greger IH
通讯作者：
Greger IH