IMMUNOLOCALIZATION OF CENTRIN DURING FERTILIZATION AND THE FIRST CELL CYCLE IN FUCUS DISTICHUS AND PELVETIA COMPRESSA (FUCALES, PHAEOPHYCEAE) 1
IMMUNOLOCALIZATION OF CENTRIN DURING FERTILIZATION AND THE FIRST CELL CYCLE IN FUCUS DISTICHUS AND PELVETIA COMPRESSA (FUCALES, PHAEOPHYCEAE) 1
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墨角藻和 Pelvetia compresssa（墨角藻目，褐藻纲）受精和第一个细胞周期期间 Centrin 的免疫定位 1
DOI：
10.1111/j.0022-3646.1997.00823.x
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发表时间：
1997
影响因子：
2.9
通讯作者：
D. Kropf
中科院分区：
文献类型：
--
作者：
S. Bisgrove;Chikako Nagasato;T. Motomura;D. Kropf
使用识别中心体相关蛋白质中心蛋白的抗体来表征中心体起源和在受精和墨角藻双列墨角藻亚种中的第一个细胞周期期间的定位。evanescens（C. Agardh）Powell和扁盾草Pelvetia compressa（J. Agardh）De Toni。在精子、卵子和受精卵的蛋白质印迹中鉴定出中心蛋白，表明该蛋白质存在于两种配子中。使用免疫荧光显微镜，centrin被发现在离散的焦点在精子。相反，卵子缺乏中心体，免疫荧光检测不到中心蛋白，表明中心蛋白可能分散在细胞质中。两个焦点的中心蛋白存在于合子的核膜，但微管仍然分散在合子核。随着第一个细胞周期的进展，中心蛋白灶在核膜上分离。微管集中在中心蛋白焦点形成中心体，在有丝分裂时产生纺锤极。
Antibodies that recognize the centrosome‐associated protein centrin were used to characterize centrosomal origin and positioning during fertilization and the first cell cycle in Fucus distichus subsp. evanescens (C. Agardh) Powell and Pelvetia compressa (J. Agardh) De Toni. Centrin was identified in sperm, eggs, and zygotes on protein blots, indicating the protein is present in both gametes. Using immunofluorescence microscopy, centrin was found in discrete foci in sperm. In contrast, eggs lack centrosomes and centrin was not detectable by immunofluorescence, indicating that centrin was probably dispersed in the cytoplasm. Two foci of centrin were present on the nuclear envelope of zygotes, but microtubules remained dispersed over the zygotic nucleus. Centrin foci separated over the nuclear envelope as the first cell cycle progressed. Microtubules became concentrated at the centrin foci to form centrosomes that gave rise to the spindle poles at mitosis.
影响因子：
2.7
作者：
Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
通讯作者：
Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
影响因子：
--
作者：
Salisbury,JL;Baron,AT;Coling,DE;Martindale,VE;Sanders,MA
通讯作者：
Sanders,MA