A Germin-Like Protein GLP1 of Legumes Mediates Symbiotic Nodulation by Interacting with an Outer Membrane Protein of Rhizobia.
A Germin-Like Protein GLP1 of Legumes Mediates Symbiotic Nodulation by Interacting with an Outer Membrane Protein of Rhizobia.
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DOI：
10.1128/spectrum.03350-22
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发表时间：
2023-02-14
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
根瘤菌可以感染豆科植物并诱导共生和防御基因的协调表达，从而建立互利共生。许多研究已经阐明了根瘤菌和寄主植物之间的分子相互作用，这些相互作用与Nod因子、胞外多糖和T3SS效应蛋白有关。然而，关于宿主植物如何识别根瘤菌的外膜蛋白（OMP）以介导共生结瘤的报道相对较少。我们前期工作中，在Mesorhizobium huakuii 7653R中发现了编码OMP的基因（Mhopa22），其同源基因广泛分布于根瘤菌目中。在这项研究中，在紫云英中鉴定出了与 Mhopa22 相互作用的种芽蛋白样蛋白 GLP1。 AsGLP1 的 RNA 干扰会导致中华北蠹毛状根中根瘤数量的减少，而 AsGLP1 的过表达则增加了根瘤的数量。在蒺藜苜蓿中，MtGLPx（参见 medtr7g111240.1，AsGLP1 的同源体）过表达或 Tnt1 突变体（glpx-1）与苜蓿中华根瘤菌 1021 共生，鉴定出一致的共生表型。glpx-1 突变体表现出过度感染并形成更多感染线，但根瘤减少。 RNA测序分析表明，许多差异表达基因参与激素信号传导和共生。总而言之，AsGLP1 及其同源物通过与根瘤菌的 OMP 相互作用，在介导早期共生过程中发挥着重要作用。重要性 这项研究是第一份描述豆类宿主植物蛋白感知根瘤菌外膜蛋白 (OMP) 并与之相互作用的报告。可以推测，GLP1通过与根瘤菌的OMP相互作用，在介导早期共生过程中发挥着重要作用。这些结果为豆科植物共生信号通路识别根瘤菌 OMP 的分子相互作用机制提供了更深入的理解和新颖的见解。我们的研究结果也可能为改善豆科植物的共生相容性和结瘤提供新的视角。
Rhizobia can infect legumes and induce the coordinated expression of symbiosis and defense genes for the establishment of mutualistic symbiosis. Numerous studies have elucidated the molecular interactions between rhizobia and host plants, which are associated with Nod factor, exopolysaccharide, and T3SS effector proteins. However, there have been relatively few reports about how the host plant recognizes the outer membrane proteins (OMPs) of rhizobia to mediate symbiotic nodulation. In our previous work, a gene (Mhopa22) encoding an OMP was identified in Mesorhizobium huakuii 7653R, whose homologous genes are widely distributed in Rhizobiales. In this study, a germin-like protein GLP1 interacting with Mhopa22 was identified in Astragalus sinicus. RNA interference of AsGLP1 resulted in a decrease in nodule number, whereas overexpression of AsGLP1 increased the number of nodules in the hairy roots of A. sinicus. Consistent symbiotic phenotypes were identified in Medicago truncatula with MtGLPx (refer to medtr7g111240.1, the isogeny of AsGLP1) overexpression or Tnt1 mutant (glpx-1) in symbiosis with Sinorhizobium meliloti 1021. The glpx-1 mutant displayed hyperinfection and the formation of more infection threads but a decrease in root nodules. RNA sequencing analysis showed that many differentially expressed genes were involved in hormone signaling and symbiosis. Taken together, AsGLP1 and its homology play an essential role in mediating the early symbiotic process through interacting with the OMPs of rhizobia. IMPORTANCE This study is the first report to characterize a legume host plant protein to sense and interact with an outer membrane protein (OMP) of rhizobia. It can be speculated that GLP1 plays an essential role to mediate early symbiotic process through interacting with OMPs of rhizobia. The results provide deeper understanding and novel insights into the molecular interactive mechanism of a legume symbiosis signaling pathway in recognition with rhizobial OMPs. Our findings may also provide a new perspective to improve the symbiotic compatibility and nodulation of legume.
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影响因子：
11.6
作者：
Chen, Tao;Zhu, Hui;Zhang, Zhongming
通讯作者：
Zhang, Zhongming
影响因子：
7.4
作者：
Arrighi, Jean-Francois;Barre, Annick;Gough, Clare
通讯作者：
Gough, Clare
影响因子：
4.8
作者：
Abdian, Patricia L.;Caramelo, Julio J.;Zorreguieta, Angeles
通讯作者：
Zorreguieta, Angeles
DOI：
10.1007/978-1-4939-8633-0_3
发表时间：
2018-01-01
期刊：
FUNCTIONAL GENOMICS IN MEDICAGO TRUNCATULA: METHODS AND PROTOCOLS
影响因子：
--
作者：
Burks, David;Azad, Rajeev;Dickstein, Rebecca
通讯作者：
Dickstein, Rebecca
影响因子：
3.2
作者：
Foreman, Dallas L.;Vanderlinde, Elizabeth M.;Yost, Christopher K.
通讯作者：
Yost, Christopher K.