Differential expression of Cosmc, T-synthase and mucins in Tn-positive colorectal cancers.
Differential expression of Cosmc, T-synthase and mucins in Tn-positive colorectal cancers.
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DOI：
10.1186/s12885-018-4708-8
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发表时间：
2018-08-16
期刊：
影响因子：
3.8
通讯作者：
Cummings RD
中科院分区：
文献类型：
--
作者：
Sun X;Ju T;Cummings RD
The Tn neoantigen (GalNAcα1-O-Ser/Thr) is an O-glycan expressed in various types of human cancers. Studies in several Tn-expressing cancer cell lines and pancreatic tumors have identified loss of Cosmc expression caused by either mutations or promoter hypermethylation. In this study, we explored the mechanism(s) for Tn expression in human colorectal cancers (CRC). Tn-expressing cell populations were isolated from CRC cell lines by Fluorescence-associated cell sorting (FACS). The expression of the Tn and sialylated Tn (STn) antigens, Cosmc, T-synthase, and mucins was characterized in paired specimens with CRC and in CRC cell lines by immunostaining, western blot, and qPCR. Using well-defined monoclonal antibodies, we confirmed prevalent Tn/STn expression in CRC samples. However, a majority of these tumors had elevated T-synthase activity and expression of both Cosmc and T-synthase proteins. Meanwhile, Tn antigen expression was not caused by mucin overproduction. In addition, we found that Tn-expressing CRC cell lines had either loss-of-function mutations in Cosmc or reversible Tn antigen expression, which was not caused by the deficiency of T-synthase activity. Our results demonstrate multiple mechanisms for Tn expression in CRCs. The online version of this article (10.1186/s12885-018-4708-8) contains supplementary material, which is available to authorized users.
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DOI：
10.1002/prca.201300024
发表时间：
2013-10
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Ju T;Wang Y;Aryal RP;Lehoux SD;Ding X;Kudelka MR;Cutler C;Zeng J;Wang J;Sun X;Heimburg-Molinaro J;Smith DF;Cummings RD
通讯作者：
Cummings RD
影响因子：
--
作者：
Huang MJ;Hu RH;Chou CH;Hsu CL;Liu YW;Huang J;Hung JS;Lai IR;Juan HF;Yu SL;Wu YM;Huang MC
通讯作者：
Huang MC
DOI：
10.1073/pnas.0402088101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Koike, T;Kimura, N;Kanangi, R
通讯作者：
Kanangi, R
影响因子：
4.8
作者：
Hassinen, Antti;Pujol, Francois M.;Kellokumpu, Sakari
通讯作者：
Kellokumpu, Sakari
影响因子：
7.7
作者：
Huang HH;Hassinen A;Sundaram S;Spiess AN;Kellokumpu S;Stanley P
通讯作者：
Stanley P