The fosfomycin resistance gene fosB3 is located on a transferable, extrachromosomal circular intermediate in clinical Enterococcus faecium isolates.
The fosfomycin resistance gene fosB3 is located on a transferable, extrachromosomal circular intermediate in clinical Enterococcus faecium isolates.
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DOI：
10.1371/journal.pone.0078106
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Xu X;Chen C;Lin D;Guo Q;Hu F;Zhu D;Li G;Wang M
来自中国的一些 VanM 型耐万古霉素屎肠球菌也对磷霉素耐药。为了研究这些临床分离株的磷霉素耐药机制，我们进行了药敏试验、过滤交配、Illumina/Solexa测序、反向PCR和磷霉素耐药基因克隆。三种屎肠球菌临床分离株对磷霉素和万古霉素高度耐药，最低抑菌浓度 (MIC) 分别 >1024 µg/ml 和 >256 µg/ml。这些菌株的磷霉素和万古霉素抗性可以通过接合共同转移。它们携带磷霉素抗性基因 fosB，编码的蛋白质与葡萄球菌质粒上编码的 FosB 有一个或两个氨基酸不同。因此，该基因被命名为fosB3。将fosB3基因克隆到pMD19-T中，并转化到大肠杆菌DH5α中。具有fosB3的转化体的磷霉素MIC比没有fosB3的转化体高750倍。 fosB3基因可以通过染色体外环状中间体转移。结果表明，fosB3基因是可转移的，可以在革兰氏阳性菌和革兰氏阴性菌中介导高水平的磷霉素抗性，并且可以位于环状中间体上。
Some VanM-type vancomycin-resistant Enterococcus faecium isolates from China are also resistant to fosfomycin. To investigate the mechanism of fosfomycin resistance in these clinical isolates, antimicrobial susceptibility testing, filter-mating, Illumina/Solexa sequencing, inverse PCR and fosfomycin resistance gene cloning were performed. Three E. faecium clinical isolates were highly resistant to fosfomycin and vancomycin with minimal inhibitory concentrations (MICs) >1024 µg/ml and >256 µg/ml, respectively. The fosfomycin and vancomycin resistance of these strains could be co-transferred by conjugation. They carried a fosfomycin resistance gene fosB encoding a protein differing by one or two amino acids from FosB, which is encoded on staphylococcal plasmids. Accordingly, the gene was designated fosB3. The fosB3 gene was cloned into pMD19-T, and transformed into E. coli DH5α. The fosfomycin MIC for transformants with fosB3 was 750-fold higher than transformants without fosB3. The fosB3 gene could be transferred by an extrachromosomal circular intermediate. The results indicate that the fosB3 gene is transferable, can mediate high level fosfomycin resistance in both Gram-positive and Gram-negative bacteria, and can be located on a circular intermediate.
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影响因子：
3.2
作者：
KADNER, RJ;WINKLER, HH
通讯作者：
WINKLER, HH
影响因子：
9.4
作者：
TENOVER, FC;ARBEIT, RD;SWAMINATHAN, B
通讯作者：
SWAMINATHAN, B
影响因子：
3.2
作者：
Christie-Oleza, J. A.;Lanfranconi, M. P.;Bosch, R.
通讯作者：
Bosch, R.
DOI：
10.1111/j.1749-6632.1974.tb43277.x
发表时间：
1974-01-01
影响因子：
5.2
作者：
KAHAN, FM;KAHAN, JS;KROPP, H
通讯作者：
KROPP, H
影响因子：
3.2
作者：
Christie-Oleza, J. A.;Nogales, B.;Bosch, R.
通讯作者：
Bosch, R.