SARS-CoV-2-specific T cells in unexposed adults display broad trafficking potential and cross-react with commensal antigens.
SARS-CoV-2-specific T cells in unexposed adults display broad trafficking potential and cross-react with commensal antigens.
复制标题
DOI：
10.1126/sciimmunol.abn3127
复制
发表时间：
2022-10-21
影响因子：
24.8
通讯作者：
Su, Laura F.
中科院分区：
文献类型：
--
作者：
Bartolo, Laurent;Afroz, Sumbul;Pan, Yi-Gen;Xu, Ruozhang;Williams, Lea;Lin, Chin-Fang;Tanes, Ceylan;Bittinger, Kyle;Friedman, Elliot S.;Gimotty, Phyllis A.;Wu, Gary D.;Su, Laura F.
T 细胞的基线组成直接影响随后对病原体的反应，但前体状态的复杂性仍然不明确。在这里，我们检查了未暴露个体中 SARS-CoV-2 特异性 T 细胞的基线状态。通过 II 类肽-MHC 四聚体染色和富集，在大流行前的血液样本中鉴定出了 SARS-CoV-2 特异性 CD4+ T 细胞。我们的数据揭示了大量表达记忆表型标记的 SARS-CoV-2 特异性 T 细胞。综合表型分析证明了多种预先存在的记忆状态，其中包括具有不同极化状态和向屏障组织运输潜力的细胞。由四聚体标记细胞产生的 T 细胞克隆与皮肤和胃肠道共生细菌的抗原发生交叉反应。对一个刺突特异性群体的直接离体四聚体染色显示出与地方性冠状病毒和共生细菌序列相似水平的交叉反应性。这些数据凸显了前体 T 细胞库的复杂性，并表明非感染性接触常见微生物是形成人类对 SARS-CoV-2 已有免疫力的关键因素。预先存在的针对 SARS-CoV-2 的免疫力表现出不同的表型，具有广泛的组织向性，并与共生抗原发生交叉反应。
The baseline composition of T cells directly impacts later response to pathogens, but the complexity of precursor states remains poorly defined. Here, we examined the baseline state of SARS-CoV-2-specific T cells in unexposed individuals. SARS-CoV-2-specific CD4+ T cells were identified in pre-pandemic blood samples by class II peptide-MHC tetramer staining and enrichment. Our data revealed a substantial number of SARS-CoV-2-specific T cells that expressed memory phenotype markers. Integrated phenotypic analyses demonstrated diverse pre-existing memory states that included cells with distinct polarization states and trafficking potential to barrier tissues. T cell clones generated from tetramer-labeled cells cross-reacted with antigens from commensal bacteria in the skin and gastrointestinal tract. Direct ex vivo tetramer staining for one spike-specific population showed a similar level of cross-reactivity to sequences from endemic coronavirus and commensal bacteria. These data highlight the complexity of precursor T cell repertoire and implicate non-infectious exposures to common microbes as a key factor that shapes human pre-existing immunity to SARS-CoV-2. Pre-existing immunity to SARS-CoV-2 displayed diverse phenotypes, had broad tissue tropism and cross-reacted with commensal antigens.
登录
查看更多内容
影响因子：
6.7
作者：
Johansson AM;Malhotra U;Kim YG;Gomez R;Krist MP;Wald A;Koelle DM;Kwok WW
通讯作者：
Kwok WW
影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
64.5
作者：
Levine JH;Simonds EF;Bendall SC;Davis KL;Amir el-AD;Tadmor MD;Litvin O;Fienberg HG;Jager A;Zunder ER;Finck R;Gedman AL;Radtke I;Downing JR;Pe'er D;Nolan GP
通讯作者：
Nolan GP
DOI：
10.1084/jem.20011502
发表时间：
2002-01-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Campbell DJ;Butcher EC
通讯作者：
Butcher EC
危及生命的Covid-19患者中针对I型IFN的自身抗体。
DOI：
10.1126/science.abd4585
发表时间：
2020-10-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bastard P;Rosen LB;Zhang Q;Michailidis E;Hoffmann HH;Zhang Y;Dorgham K;Philippot Q;Rosain J;Béziat V;Manry J;Shaw E;Haljasmägi L;Peterson P;Lorenzo L;Bizien L;Trouillet-Assant S;Dobbs K;de Jesus AA;Belot A;Kallaste A;Catherinot E;Tandjaoui-Lambiotte Y;Le Pen J;Kerner G;Bigio B;Seeleuthner Y;Yang R;Bolze A;Spaan AN;Delmonte OM;Abers MS;Aiuti A;Casari G;Lampasona V;Piemonti L;Ciceri F;Bilguvar K;Lifton RP;Vasse M;Smadja DM;Migaud M;Hadjadj J;Terrier B;Duffy D;Quintana-Murci L;van de Beek D;Roussel L;Vinh DC;Tangye SG;Haerynck F;Dalmau D;Martinez-Picado J;Brodin P;Nussenzweig MC;Boisson-Dupuis S;Rodríguez-Gallego C;Vogt G;Mogensen TH;Oler AJ;Gu J;Burbelo PD;Cohen JI;Biondi A;Bettini LR;D'Angio M;Bonfanti P;Rossignol P;Mayaux J;Rieux-Laucat F;Husebye ES;Fusco F;Ursini MV;Imberti L;Sottini A;Paghera S;Quiros-Roldan E;Rossi C;Castagnoli R;Montagna D;Licari A;Marseglia GL;Duval X;Ghosn J;HGID Lab;NIAID-USUHS Immune Response to COVID Group;COVID Clinicians;COVID-STORM Clinicians;Imagine COVID Group;French COVID Cohort Study Group;Milieu Intérieur Consortium;CoV-Contact Cohort;Amsterdam UMC Covid-19 Biobank;COVID Human Genetic Effort;Tsang JS;Goldbach-Mansky R;Kisand K;Lionakis MS;Puel A;Zhang SY;Holland SM;Gorochov G;Jouanguy E;Rice CM;Cobat A;Notarangelo LD;Abel L;Su HC;Casanova JL
通讯作者：
Casanova JL