Müllerian duct maintenance in heterotypic organ culture after in vivo exposure to diethylstilbestrol.
Müllerian duct maintenance in heterotypic organ culture after in vivo exposure to diethylstilbestrol.
复制标题
体内暴露于己烯雌酚后异型器官培养中苗勒氏管的维持。
DOI：
10.1210/endo-115-5-1863
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发表时间：
1984
期刊：
影响因子：
4.8
通讯作者：
John A. McLachlan
中科院分区：
文献类型：
--
作者：
R. Newbold;Yoshihide Suzuki;John A. McLachlan
产前暴露于己烯雌酚(DES)会导致雄性后代Müllerian导管持续存在。在不同导管(胚胎生殖道)与处理或对照胚胎睾丸共培养的器官培养系统中，研究了DES对Müllerian管退化的影响。孕期第9天至第12天，母亲皮下注射DES(100微克/千克体重)。于妊娠第13天取胚胎组织培养72h，在器官培养中，当对照生殖道与对照睾丸联合时，发生了与体内相似的缪勒管退化。然而，当DES处理的睾丸和DES处理的生殖道一起培养时，在100%的组织中观察到缪勒管的维持。当对照生殖道和DES处理的睾丸结合形成重组时，苗勒管退化(87%)。然而，在DES处理的生殖道和对照睾丸的组合中，41%的培养组织显示Müllerian管部分消退，59%的组织没有消退。这些数据支持了以前的活体实验结果，即产前暴露于DES对苗勒管退行性变有抑制作用，并进一步表明这种抑制作用主要是由于处理过的胚胎苗勒管的反应性降低所致。
Prenatal exposure to diethylstilbestrol (DES) results in the persistence of Müllerian ducts in male offspring. The influence of DES on Müllerian duct regression was studied using an organ culture system in which DES-treated or control indifferent ducts (embryonic reproductive tracts) were cocultured along with treated or control embryonic testes. Prenatal DES exposure was by sc injection of the mother (100 micrograms/kg BW) on days 9 through 12 of gestation. Embryonic tissues were removed on day 13 of gestation and cultured for 72 h. In organ culture, Müllerian duct regression, comparable to that seen in vivo, occurred when control reproductive tracts were associated with control testes. However, maintenance of the Müllerian duct was observed in 100% of the tissues when DES-treated testes and DES-treated reproductive tracts were cultured together. When recombinations were formed by the association of control reproductive tracts and DES-treated testes, there was regression of the Müllerian duct (87%). However, in the combinations of DES-treated reproductive tracts and control testes, 41% of the cultured tissue demonstrated partial regression of the Müllerian duct, and 59% showed no regression. These data support previous in vivo results that prenatal exposure to DES has an inhibitory effect on Müllerian duct regression and further suggest that this inhibitory effect is mainly due to a decrease in responsiveness of the treated embryonic Müllerian duct.
核苷酸焦磷酸酶在苗勒氏管消退中的作用。
影响因子：
2.7
作者：
Fallat,ME;Hutson,JM;Budzik,GP;Donahoe,PK
通讯作者：
Donahoe,PK
苗勒氏管消退过程中的磷酸化事件。
DOI：
10.1126/science.6607531
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hutson,JM;Fallat,ME;Kamagata,S;Donahoe,PK;Budzik,GP
通讯作者：
Budzik,GP